This study examined the effect of two different sport drink supplementation (Northforce Ltd., Kuusamo, Finland) on the acute immune responses in recreationally trained non-athletes after standardized 20 km (men) / 18 km (women) runs. The runs were performed on an indoor 200 m track at 75% of maximal oxygen uptake (VO 2max ) using one liter of carbohydrate (CHO) supplementation (7% of CHO) or CHOPROT (low CHO 1.5 % and protein 40 mg) in a randomized, double-blind, placebo (PLA) controlled design. Seven subjects (four males and three females) were able to finish all three trials. Blood samples were obtained at rest (pre) and immediately after the cessation of the run (post). The total and differential white blood cells (WBC), cortisol (COR), C-reactive protein (CRP) and interleukin-6 (IL-6) were analyzed. The run task induced significant (p<0.05) increases in WBC, neutrophil and interleukin-6 concentrations in every trial. The loading did not have significant effect on lymphocyte count or CRP. There was a significant (p<0.05) increase in cortisol with PLA and CHOPROT but not with CHO. Post-run increases in plasma concentrations of WBC and neutrophil were significantly lower with CHO compared to PLA (p<0.05). The IL-6 concentrations did not differ significantly between the trials. The data indicate that carbohydrate ingestion with a typical concentration (7 % of CHO) found in sport drink attenuates cortisol and WBC concentrations in response to high intensity long distance running. The sport drink with a lower level of CHO (1.5 %) with a small (40 mg) amount of protein did not differ significantly from PLA in the measured responses.