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Acta Physiologica 2012; Volume 205, Supplement 690
Joint Meeting of the Hungarian Biophysical Society, Hungarian Physiological Society, Hungarian Society of Anatomists and Hungarian Society of Microcirculation & Vascular Biology
6/11/2012-6/13/2012
Debrecen, Hungary
EXAMINATION OF PACAP IMMUNOREACTIVITY IN DIFFERENT MILK AND INFANT FORMULA SAMPLES AND PRESENCE OF PAC1 RECEPTOR IN THE MAMMARY GLAND DURING LACTATION
Abstract number: P5
Csanaky1 K, Banki1 E, Szabadfi2 K, Tarcai3 I, Czegledi4 L, Helyes5 Zs, Mark6 L, Zapf7 I, Szanto7 Z, Pirger8 Zs, Naskar9 S, Pirger9 Zs, Reglodi1 D, Tamas1 A
1Department of Anatomy, PTE-MTA "Lendulet PACAP Research Group, University of Pecs, Pecs, Hungary
2Department of Experimental Zoology and Neurobiology, University of Pecs, Pecs, Hungary
3Unified Health Institutions, Pecs
4Institute of Animal Science, Centre for Agricultural and Applied Economic Sciences, University of Debrecen, Debrecen, Hungary
5Department Pharmacology and Pharmacotherapy University of Pecs, Pecs, Hungary
6Department of Biochemistry and Medical Chemistry, University of Pecs, Pecs, Hungary
7Surgery Clinic, University of Pecs, Pecs, Hungary
8Comparative Neurobiological Research Group, Department of Experimental Zoology, Balaton Limnological Research Institute, Hungarian Academy of Sciences, Tihany, Hungary
9School of Life Sciences, University of Sussex, Falmer, Brighton, United Kingdom
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide with widespread occurrence in the nervous system and peripheral organs, including the mammary gland. Previously, we have shown that PACAP38 is present in the human milk at higher levels than in respective blood samples. However, it is not known how PACAP levels and the expression of PAC1 receptor change during lactation. Therefore, the aim of our study was to investigate PACAP38-like immunoreactivity (PACAP38-LI) in human colostrum, transitional and mature milk during lactation and to compare the expression of PAC1 receptors in lactating and non-lactating mammary glands. In the second part of the study we examined the PACAP38-LI in the cow milk and cow milk-based infant formulas and milk powder samples with RIA and mass spectrometry. We found that PACAP38-LI was significantly higher in human colostrums samples than in the transitional and mature milk. PACAP38-LI did not show any significant changes within the first 10-month-period of lactation, but a significant increase was observed thereafter, up to the examined 17th months. Weak expression of PAC1 receptors was detected in non-lactating sheep and human mammary glands, but a significant increase was observed in the lactating sheep samples. We found similar PACAP38-LI in cow milk and cow-milk based formulas with RIA and we could also detect PACAP38 in some of the infant formula samples. We also provide evidence for the PACAP content of milk powder with indirect Western-blot analysis. In summary, the present study is the first to show changes of PACAP levels in human milk during lactation. The presence of PACAP in the milk suggests a potential role in the development of newborn, while the increased expressions of PAC1 receptors on lactating breast may indicate a PACAP38/PAC1 interaction in the mammary gland during lactation.
Supported by OTKA (K72592, 73044, CNK78480), TAMOP (4.2.1.B-10/2/KONV-2010-002, 4.2.2.B-10/1-2010-0029, 4.2.1/B-09/1/KONV-2010-0007), Bolyai Scholarship, Richter Foundation, PTE AOK
Research Grant KA- 34039/10-26, KA-34039/10-10, PTE-MTA "Lendulet" and the European Social Fund.
To cite this abstract, please use the following information:
Acta Physiologica 2012; Volume 205, Supplement 690 :P5