We have documented that the human salivary glucose comes mainly from salivary glands (parotid, sub-maxillary and sublingual). The primary saliva is isotonic and becomes hypotonic during its transport to the oral cavity because of the reabsorption of sodium by ductal cells. Therefore, this study aims, by analogy to enterocytes, to assess whether if the glucose secreted by acinar cells was reabsorbed by ductal cells. Quantitative polymerase chain reaction was used to measure mRNA expression of GLUT4 in different salivary glands. Total RNA from salivary glands was extracted and subjected to mRNA quantification using specific primers for SGLT1 and Glut4 and SYBR Green on a LightCycler 480. Anti-GLUT4 antibody (Millipore 07–1404, 1/500) and anti-SGLT1 antibody (Millipore 07–1417, 1/500) were applied on the fixed salivary glands sections. Staining included avidin-biotin-peroxidase (Vector Labs, Belgium) and diaminobenzidine (Dako, Belgium), with hematoxylin counterstaining. The presence of SGLT1 was detected in rat parotid and sub-maxillary ductal cells by immunehistochemistry. SGLT1 is also present in human parotid ductal cells. Moreover, we have discovered the presence of GLUT4 in ductal cells from human parotid, and both rat parotid and sub-maxillary glands. The presence of both SGLT1 and GLUT4 was also confirmed by qRT-PCR in theses salivary glands. In conclusion this study documents the presence of SGLT1 and GLUT4 in ductal cells of human parotid and rat parotid and sub-maxillary glands. We postulate that their presence in ductal cells of salivary glands may allow the reabsorption of glucose secreted by acinar cells of salivary glands. Further experiments are required to assess the validity of this proposal by co-localization immunohistochemistry.