Back
Acta Physiologica 2011; Volume 203, Supplement 686
Joint Congress of FEPS and Turkish Society of Physiological Sciences
9/3/2011-9/7/2011
Istanbul, Turkey
TRANSGELIN IDENTIFIED IN SEMINAL VESICLES OF SAND RAT PSAMMOMYS OBESUS DURING THE BREEDING SEASON
Abstract number: PC004
Naima1 Kaci Ouchfoun, Abdelkrim2 Boudrissa, Bekkouche Fatima3 Hadj, Spychalowicz Therese1 Gernigon
1Laboratoire des Zones Arides, Faculte des Sciences Biologiques, USTHB, BP 132 El Alia, 16100 Alger, Algeria
2Service de Parasitologie, Institut Pasteur dAlgerie, 01 Rue du Docteur Laveran, El Hamma, Alger, Algeria
33Laboratoire de Biologie et Physiologie des Organismes, FSB/USTHB, BP 132 El Alia, 16100 Alger, Algeria
Objective:
The aim of this study was to investigate immunolocalization and identification of POSVP21 in seminal vesicles of sand rat Psammomys obesus.
Methods:
Using a rabbit polyclonal antibodies preparation against POSVP21, we demonstrated by the avidin-biotin peroxidase procedure and the whole RNA from seminal vesicles translation the site synthesis localization of POSVP21.
Results:
POSVP21 is localized in the cytoplasm of epithelial cells and in secretory products in the lumen. To identify POSVP21, two dimensional gel analysis and tandem mass spectrometry (nanoLC-MS/MS) was performed. POSVP21 is separated on pH-gradient electrophoresis into at least 4 variants a, b, c and d with similar apparent Mw and pHi values varying from 4 to 7. Peptides sequences analysis of POSVP21 showed that it has a similar amino acid composition to the "Transgelin" peptide sequence from the rat Rattus norvegicus.
Conclusions:
We conclude that POSVP21 is a similar protein to the transgelin from the rat Rattus norvegicus. "Transgelin" is identified for the first time within sand rat Psammomys obesus.
To cite this abstract, please use the following information:
Acta Physiologica 2011; Volume 203, Supplement 686 :PC004