Previous studies have indicated that mineralocorticoid receptors (MR) and glucocorticoid receptors (GR) enhance memory consolidation, but their effects on memory reconsolidation are not clear. In this study, we studied the role of MR and GR on memory reconsolidation. Sixty young rats were trained in a passive avoidance task. For memory reactivation, rats were returned to the chamber 48 h later. Immediately after reactivation, rats were injected with MR antagonist spironolactone (50 mg/kg) or GR antagonist RU38486 or spironolactone + RU38486. Control animals received vehicle. Two, four, and seven days after memory reactivation, rats were returned to the context for 10 min, and step-through latency was recorded. The results showed that administration of spironolactone or RU38486 or combination of both induced impairment of memory reconsolidation. Further, the results showed that drugs deficit was not reversed by a reminder shock. These findings provide evidence that blockade of glucocorticoid and mineralocorticoid receptors impair memory reconsolidation.