Exercise is a prototype of physical stressor that induces a host of cardiovascular, hormonal, metabolic and immunological changes, whose provoking mechanisms is still a topical issue. The aim of this study was to estimate the impact of physical activity on changes in vasomotor reactions (microvascular beds of the skin) and concentrations of blood biomarkers (cytokines, hormones) in healthy humans with different physical condition. There were 58 (48 men; 10 women) young, healthy individuals participating in this study. These individuals, who had different physical condition, were formed in age and body mass index matched study groups (p>0,05). The laser Doppler flow technique (MoorLDI2) was used to estimate the regional blood flow changes on the dorsum of the foot induced by local heating, Ach and SNP; assessment of exercise (1,5 – 2h) induced changes in concentrations of cytokines and hormones were made with flow cytometry (Luminex 200, Immulite 2500). There are no significant differences between volleyball players and control group in laser dopplerography tests. Exercise influences cytokine concentrations in different ways – increase in IL-6, MCP-1, decrease in IL-8, TNF-a and no changes in leptin concentrations, but stress hormone concentrations raise in all research participant groups. There are interconnections between exercise induced cytokine and hormone concentrations – IL-8 together with stress hormones is substantially associated with IL-6. There is correlation between acetylcholine induced vasodilation (endothelium-dependent) and stress hormones and this vasodilation maximum is substantially associated with stress hormones (cortisol, adrenocorticotrophic hormone) and cytokines. In conclusion physical activity have an impact on blood biomarkers (cytokines, hormones) changes that probably describe energy assurance of working muscles growing needs.