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Acta Physiologica 2009; Volume 195, Supplement 670
Belgian Society for Fundamental and Clinical Physiology and Pharmacology, Spring Meeting 2009
3/7/2009-3/7/2009
Ghent University, Gent, Belgium
LOCAL INFUSION OF NEUROPEPTIDE Y HAS ANTI-EPILEPTIC EFFECTS IN FULLY KINDLED RATS
Abstract number: P-11
Dauwe1 I., Raedt1 R., Van Dycke1 A., Meurs1 A., Wyckhuys1 T., El Tahry1 R., Vonck1 K., Wadman2 W., Boon1 P.
1Laboratory of Clinical and Experimental Neurophysiology, Department of Neurology, Ghent University Hospital, Ghent, Belgium
2Swammerdam Institute of Life Sciences, Department Neurobiology, University of Amsterdam, Amsterdam, The Netherlands
Purpose:
Due to the high number of medically untreatable patients, developing new therapies for temporal lobe epilepsy remains a challenge. Local delivery of anti-epileptic substances in the ictal onset region is a promising technique because of its benefit that systemic side effects can be avoided. In this study we evaluate the anti-epileptic effect of local delivery of neuropeptide Y (NPY) in the hippocampus of kindled rats.
Methods:
Rats (n=11) were implanted with a bipolar registration electrode in the left hippocampus and a stimulation electrode-cannula complex in the right hippocampus. Once the rats were fully kindled, the cannula was connected to an osmotic minipump to perform continuous intrahippocampal delivery (0.47 ml/h). A cross-over experiment was performed to compare the effect of local delivery of NPY (2 mg/ml) and saline on kindling characteristics. On every other day during six days of local delivery, afterdischarge threshold, afterdischarge duration and seizure severity were evaluated.
Results:
Comparison of NPY treatment and saline delivery revealed a significant decrease in seizure severity (p <0.01) and a significant reduction of afterdischarge duration (p <0.01). No significant difference was seen in afterdischarge threshold (NPY 542 mA 68; saline 389 mA 74).
Conclusion:
This study proves the anti-epileptic effect of continuous intrahippocampal delivery of neuropeptide Y in fully kindled rats.
To cite this abstract, please use the following information:
Acta Physiologica 2009; Volume 195, Supplement 670 :P-11