The activity of the amiloride- sensitive epithelial sodium channel ENaC is considered to establish a crucial element for the maintenance of sodium homeostasis in mammals. Regulation of the three ENaC subunits alpha, beta, and gamma, e.g., by aldosterone has been shown at transcriptional and posttranslational levels. Marked dissociation between mRNA- and protein levels in ENaC expression studies suggests that mRNA- specific mechanisms, such as changes of mRNA stability or in the efficiency of translation, are involved as well., These are mainly mediated by interaction of RNA- binding proteins (RNA- BP) with untranslated regions (UTR) of mRNAs. Theoretical analysis of ENaC UTRs revealed an AU-rich element (ARE) in the gamma ENaC- 3*UTR indicating a potential role of ARE-BPs for posttranscriptional control of ENaC expression. To investigate this issue we studied possible interactions with ENaC- UTRs by RNA- affinity- chromatography, identified RNA-BPs by MALDI- TOF-MS and UV-crosslinking, polysome gradient analysis, and performed co-expression experiments using luciferase- ENaC- UTR constructs and RNA- BP plasmid. Our results demonstrate that several RNA-BP interact with the RNA of the ENaC subunits, in particular with the gamma- ENaC- 3*UTR.