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Acta Physiologica 2009; Volume 195, Supplement 669
The 88th Annual Meeting of The German Physiological Society
3/22/2009-3/25/2009
Giessen, Germany
VISUALISATION OF RESPIRATORY ACTIVITY IN BREATHING SLICES FROM MICE USING VOLTAGE-SENSITIVE DYES
Abstract number: O13
Hulsmann1 S., Masumiya2 H., Oku3 Y.
1Abt. Neuro- und Sinnesphysiologie, Zentrum Physiologie und Pathophysiologie, Gttingen
2Department of Physiology, Hyogo College of Medicine,, Nishinomiya, Japan
3Department of Physiology, Hyogo College of Medicine,, Hyogo, Japan
The investigation of the spatial sequencing of neuronal activity in the respiratory network during normal and impaired activity requires high temporal resolution. Voltage sensitive dyes meet these requirements and can be used to identify the exact timing of activity in different elements and regions of the respiratory network.
We have been able to established voltage imaging in the rhythmic slice preparation from the pre-Bötzinger complex of mice. In this slice preparation we have used di-4-ANEPPS. Recordings were performed at an unprecedented speed of 100Hz and above using spike triggered averaging with a CMOS-camera (MiCAM Ultima, Japan). The speed of the image acquisition was fast enough to show that the activity in the pre-Bötzinger complex is preceding the activity in the hypoglossal nucleus.
We conclude that voltage imaging in slice preparation is a valuable tool to analyze changes of spatial pattern in different mouse models of respiratory disturbances.
Supported by the Japan Society for the Promotion of Science
To cite this abstract, please use the following information:
Acta Physiologica 2009; Volume 195, Supplement 669 :O13