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Acta Physiologica 2009; Volume 195, Supplement 667
XXXV Congress of The Spanish Society for Physiological Sciences
2/17/2009-2/20/2009
Valencia, Spain
OXIDATIVE STRESS IS PRESENT IN PRIMARY FIBROBLASTS FROM A KINDLER SYNDROME PATIENT
Abstract number: P110
Mora1,2 NJ, Garcia-Gimenez2 JL, Markovic1,2 J, Dasi3 F, Gimeno2 A, Lloret1,2 A, De la Concepcion1 N, Perez-Quilis2 C, Velazquez1 A, Garcia1 G, Del Rio4 M, Pallardo1,2 FV
1Department of Physiology, Medical School of the University of Valencia, Blasco Ibaez 15, 46010, Valencia, Spain.
2CIBERER, Blasco Ibez, 15, 46010, Valencia, Spain.
3Unidad Central de Investigacion, Medical School of the University of Valencia, Spain.
4Dep. Investigacin Basica. CIEMAT. [email protected]
Aim:
To determine the oxidative status in a cellular model from Kindler syndrome patients.
Methods:
Cell proliferation level by cell counting, hydrogen peroxide and superoxide by flow cytometry and fluorimetry, glutathione determination by H.P.L.C. and enzymatic essay; antioxidant enzymes expression was evaluated by RT-PCR and western blotting analysis.
Results:
The cells originating from a Kindler patient showed pro apoptotic morphology. The oxidative stress parameters: GSSG and GSSG/GSH ratio, as well as superoxide levels demonstrated a moderate increase. In addition, the antioxidant enzymes MnSOD and thioredoxin 1 presented a diminished levels comparing to the control.
Conclusion:
To our knowledge, this is the first indication of the impairment of the antioxidant defence in relation to Kindler syndrome.
To cite this abstract, please use the following information:
Acta Physiologica 2009; Volume 195, Supplement 667 :P110