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Acta Physiologica 2009; Volume 195, Supplement 667
XXXV Congress of The Spanish Society for Physiological Sciences
2/17/2009-2/20/2009
Valencia, Spain
DIFFERENT PATTERNS OF IN VIVO PRO-OXIDANT STATES IN A SET OF CANCER-OR AGING-RELATED GENETIC DISEASES
Abstract number: P39
Lloreta A, Calzoneb R, Dunsterc C, Maninid P, d'Ischiad M, Degane P, Kellyc FJ, Pallardoa FV, Zatteraleb A, Paganof G
aaDepartment of Physiology, University of Valencia, Avenida Blasco Ibaez 15, E-46010 Valencia, Spain
bbDepartment of Genetics, Elena dAosta Hospital, via Cagnazzi, I-80136 Naples, Italy
ccPharmaceutical Science Research Division, King's College, London SE1 9NH, UK
ddDepartment of Organic Chemistry and Biochemistry, Federico II University, I-80126 Naples, Italy
eeIST, Italian National Cancer Research Institute, I-16132 Genoa, Italy
ffItalian Association for Fanconi Anemia Research, Piazza Bovio 14, I-80133 Naples, Italy. [email protected]
Aim:
A comparative evaluation of pro-oxidant states in 82 patients with ataxia telangiectasia (AT), Bloom syndrome (BS), Down syndrome (DS), Fanconi anemia (FA), Werner syndrome (WS), and xeroderma pigmentosum (XP) vs 98 control donors.
Methods:
GSSG and GSH levels, 8-hydroxy-2'-deoxyguanosine, plasma levels of glyoxal and methylglyoxal and uric acid, ascorbic acid, a- and g-tocopherol were measured as described previously.
Results:
Leukocyte 8-OHdG levels ranked as follows: WS > BS [asymp] FA [asymp] XP > DS [asymp] AT [asymp] controls. Urinary 8-OHdG levels were significantly increased in a total of 22 patients with BS, FA, or XP vs 47 controls. The GSSG:GSH ratio was significantly increased in patients with WS and in young (<= 15 years) patients with DS or with FA and decreased in older patients with DS or FA and in AT, BS, and XP patients. The plasma levels of Glx and/or MGlx were significantly increased in patients with WS, FA, and DS. The UA and AA levels were significantly increased in WS and DS patients, but not in AT, FA, BS, nor XP patients.
Conclusion:
Our results suggest that oxidative stress may play an important role in the pathogenesis of the diseases such as AT, BS, DS, FA, WS, and XP. Notwithstanding the genotypic differences among these disorders, the examination of their phenotypic analogies may shed valuable insights into the pathogenesis of these diseases as well as of cancer and aging in general.
To cite this abstract, please use the following information:
Acta Physiologica 2009; Volume 195, Supplement 667 :P39