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Acta Physiologica 2008; Volume 194, Supplement 668
Belgian Society for Fundamental and Clinical Physiology and Pharmacology, Autumn Meeting 2008
11/1/2008-11/30/2008
Université Catholique de Louvain, Louvain-en-Woluwé, Belgium
EFFECTS OF AN ORAL SUPPLEMENTATION WITH GLUCOSAMINE AND CHONDROITIN SULPHATE ON MMP-2 ACTIVITY IN SUPERNATANTS OF IL-1 STIMULATED-EQUINE CHONDROCYTES
Abstract number: P-15
Schulsse1 S., Wiggers1 L., Daix1 M., Kirschvink1 N.
1Laboratory of Animal Physiology, Departement of Veterinary Medicine, University of Namur, Namur, Belgium.
Matrix metalloproteinases (MMP) are involved in the progress of the cartilage proteolysis occurring in joint diseases. The aim of this study was to assess the effect of an in vivo supplementation with a nutraceutical containing glucosamine (GS), chondroïtin sulphate (CS) and Harpagophytum procumbens (HP)extracts on MMP-2 activity in supernatants of IL-1b stimulated-culture chondrocytes. After six weeks of supplementation with A (GS/CS/HP) or B (placebo), articular cartilage of 14 ponies was collected post-mortem from fetlocks and chondrocyte cultures were realized. After stimulation with 2.5, 5 and 10 ng/ml IL-1b for 24h, MMP-2 activity was determined in supernatants by gelatin zymography standardized to cells and supernatants total proteins.
Mean MMP-2 activity (Arbitrary Units of O.D.) in supernatants
| | Cell proteins standardisation | Supernatant proteins standardisation |
|---|
| Groups | A (n=11) | B (n=7) | A | B |
|---|
| Control | 1.7050.977 | 1.5271.240 | 0.9650.763(n=10) | 0.8270.595(n=8) |
| IL-1b 2.5ng/ml | 4.0821.267a,b | 2.2231.107 | 3.1760.755(n=7)b | 2.3400.838(n=7)b |
| IL-1b 5ng/ml | 3.9511.436a,b | 2.4651.013 | 3.2430.819(n=8)b | 2.935 0.906(n=7)b |
| IL-1b 10ng/ml | 4.5551.547a,b | 2.9321.315b | 4.1050.808(n=8)a,b | 3.1380.573(n=6)b |
| aP values less than 0,05 are considered as significant vs group B; b P values less than 0,05 are considered as significant vs respective control |
IL-1b stimulation induced a significant dose-dependent increase of MMP-2 activity compared to the controls. After a normalisation to cell proteins, MMP-2 activity was significantly higher in IL-1b-stimulated chondrocyte cultures from group A (P<0.05 for each dose of IL-1b) whereas no significant differences between groups could be established for unstimulated control cultures (P=0.725). After a normalisation to supernatant proteins, MMP-2 activity was significantly higher in chondrocyte cultures from group A and stimulated with 10 ng/ml of IL-1b (P=0.029). Control, 2.5 ng/ml and 5ng/ml stimulation values did not differ between groups.
These study results suggest that the orally administrated neutraceutical had quantifiable effects on the MMP-2 metabolism of cultured chondrocytes. Further studies are warranted to assess the functional impact of an increased MMP-2 turnover in chondrocytes.
To cite this abstract, please use the following information:
Acta Physiologica 2008; Volume 194, Supplement 668 :P-15