Recruitment of leukocytes to the arterial wall is a key mechanism in the development of atherosclerosis. Previous histological data have shown the importance of monocytes and lymphocytes in atherosclerotic lesions, whereas the role for neutrophils remains unclear. Data from experiments using intravital microscopy have shown the efficacy of the multistep recruitment cascade of leukocytes to atherosclerotic lesions in vivo. However, no studies have previously described which subclasses of leukocytes that interact with atherosclerotic endothelium. In this study, we used intravital microscopy on various mouse models to study the contribution of individual leukocyte subclasses in interactions between leukocytes and endothelium in atherosclerosis. In atherosclerotic ApoE^-/- mice treated with an intravenous injection of rhodamine 6G (R6G), which labels all circulating leukocytes, significant rolling along aortic endothelium was observed. In ApoE^-/- mice crossed with mice carrying a knock-in mutation for EGFP in the lysozyme M locus (lys^EGFP/EGFP mice), which makes neutrophils and monocytes fluorescent, the number of interacting leukocytes was not significantly different compared to ApoE^-/- mice. In ApoE^-/- mice that were lethally irradiated and rescued with bone marrow from lys^EGFP/EGFP mice the number of rolling leukocytes on atherosclerotic endothelium were significantly higher (13.5 ± 5.4 mean±SEM) compared to ApoE^-/- transplanted with bone marrow from CX3CR1^EGFP/EGFP mice (0.39 ± 0.2 rolling cells/min; p = 0.002), this mouse strain has a knock-in mutation for EGFP in the CX3CR1 locus which makes monocytes fluorescent. Our results suggest that the majority of cells interacting with atherosclerotic endothelium are neutrophils with a minor contribution of monocytes and lymphocytes.