Information on the anaerobic threshold for each individual is important for developing aerobic training programs and monitor training progress. However, currently valid measurement of the anaerobic threshold requires expensive equipment and trained professionals. The aim of this study was to develop and test the validity of a new field test to individually estimate the anaerobic threshold. The subjects for this study were 34 volunteers, both athletes and recreational joggers. Each subject performed two tests in random order. On one occasion maximum oxygen consumption (VO₂max) and lactate threshold (4 mM) were measured according to standard protocols. On the other occasion a running test was conducted. The running test was a multi-stage test until exhaustion where each stage was 7 min and the speed increased 0.6 m/s at each stage. The running speed at exhaustion was calculated as the speed at the last fully completed stage plus the fraction of the speed at the final stage based on the time completed at that stage. The correlation coefficient between the running test outcome and the running speed at the anaerobic threshold was 0.84. However, the correlation coefficient between the running test outcome and VO₂max was 0.57. The running speed at exhaustion from the running test was 0.30 m/s (SD= 0.19 m/s) above the running speed at the anaerobic threshold and, therefore, the subjects performed, on average, a net anaerobic work of 150 m (SD= 77 m). In conclusion the running test was found to be a good predictor of the anaerobic threshold.