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Acta Physiologica 2006; Volume 187, Supplement 659
The Scandinavian Physiological Society's Annual Meeting
8/11/2006-8/13/2006
Reykjavik, Iceland
THE EFFECT OF TETRADECYLTHIOACETIC ACID (TTA) ON BLOOD PRESSURE (BP) IN TWO KIDNEYS, ONE CLIP (2K1C) HYPERTENSION
Abstract number: 0906
BIVOL1 M, HULTSTROM1 M, VAGNES1 O, BERGE1 R, IVERSEN1 B
1University of Bergen, Haukeland University Hospital, Institute of Medicine, Room 217A, Bergen, Norway [email protected]
Background: The effect of TTA, an analogue fatty acid, known as a pan PPAR ligand, was investigated in 2K1C hypertension.
Methods: 2K1C hypertension was induced by clip on the right renal artery and TTA was administered orally from the time of clipping. The BP and renal blood flow (RBF) response after 2.5 ng ANG II injection was examined before and after indomethacin (cyclooxygenase (COX) inhibitor) in the non-clipped kidney. Plasma renin activity (PRA) and ANG II plasma levels were measured. mRNA for renin, COX 1 and COX 2 were investigated by RT-PCR, Western blotting and measure of enzymes activity (EIA).
Results: Untreated 2K1C had 183 ± 4 mmHg 6 weeks after clipping, while BP in TTA treated 2K1C was 128 ± 8 mmHg (p < 0.001). PRA was increased in untreated 2K1C and was significantly reduced in TTA treated 2K1C (p < 0.001). mRNA for renin was significantly reduced in the clipped kidney of TTA treated group. RBF vascular response to ANG II was increased (p = 0.01) in 2K1C after TTA treatment. After indomethacin, RBF response was unchanged in TTA treated groups. mRNA COX 2 expression was downregulated in 2K1C group after TTA treatment. Both COX1 and COX2 activity were downregulated in TTA treated groups independent of clip, and Western Blot showed coresponding results.
Conclusion: TTA reduces BP by lowering PRA and mRNA for renin in clipped kidney. A possible mechanism may be through genetic downregulation of COX 2.
To cite this abstract, please use the following information:
Acta Physiologica 2006; Volume 187, Supplement 659 :0906