Introduction: 

Previous reports show that high level of homocysteine in the blood is related to a higher risk of coronary heart disease, stroke, atherosclerosis and peripheral vascular disease. Homocysteine is an intermediate in synthesis of methionine and it is metabolised by enzymes that are dependent on vitamin B12 and folic acid.

Aim: 

Our study had like target to establishment of a correlation between the reactive oxygen species and the intervention of homocysteine in the development of the cardiovascular disorders.

Material and Method: 

The researches were performed on 21 patients with type 2 diabetes before and after a daily oral treatment during 3 weeks with 1 mg folic acid/day and 2 mcg cyancobalamin/day. Before and after the therapy the levels of malondialdehyde (MDA) (triobarbituric acid method) carbonylated proteins (guanidine hydrochloride method), and ceruloplasmin (Ravin method) were assessed. For the assessment of blood speed in the peripheral vessels the echo Doppler method was used. The level of blood glucose, cholesterol, creatinine and triglyceride was also determined.

Results: 

The MDA is significantly raised and the treatment with folic acid and vitamin B12 is able to reduce its level, but not to the normal value. The carbonylated proteins have also a high concentration in comparison to the control group.

Conclusions: 

The treatment was not able to improve the peripheral circulation but it was efficient in the removal of the oxidative stress.