Aim: 

Acute lead intoxication induces changes at all levels of organisms metabolism, its functional and structural organisation. On cell level damage factors include damage of membranous apparatus and fermental systems. The organism is influenced with a significant amount of adverse ecological factors among which can be social stress (SS). Effects of the combined influence of social stress and plumbum-ions practically are still not studied.

Methods: 

SS predetermined by isolation of Wistar male rats (m [asymp] 200g) during 2 days, with only water in daily ration. The following 10 days of stress rats were injected with a solution of plumbum nitrate (PN). Animals of the I group received 1/20 fatal dozes (FD) of PN, the II group – 1/200 FD. All parameters were estimated in liver homogenate. A level of MDA estimated by method of Timirbylatov and Seleznev (1981), catalase (CAT) activity – by Korolyk's method (1988); concentration of NO₂- – by Green L.C. (1982), and diene conjugates (DC) – by Plocer's method (1982).

Results: 

MDA levels (mmol/l) were the highest in the I group (579.09 ± 5.08) compared to II group (451.208 ± 3.59) and control group (443.69 ± 4.09). CAT activity (mmol [H₂O₂]/min/mg): I group (10.29 ± 0.049), II group (10.11 ± 0.03), control (2.83 ± 0.06). NO₂- levels (mmol/l): I group (12.39 ± 0.27), II group (11.10 ± 0.21), control (9.76 ± 0.12). DC (c.u.): I group (0.76 ± 0.005), II group (0.706 ± 0.003), control (0.087 ± 0.008). IP: I group (0.023 ± 0.005), II group (0.032 ± 0.002), control (0.073 ± 0.007).

Conclusion: 

our data reveal activation of lipid peroxidation, which displays metabolic shifts under conditions of oxidation stress induced by heavy metals intoxication. As a result there is a compensatory activation of antioxidant enzymes (CAT) and NO hyperproduction. Decrease of IP at research group of animals confirms disbalance between processes of synthesis of free radicals and their inactivation. The received results depend on a dose of PN.