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Acta Physiologica 2007; Volume 191, Supplement 658
Joint Meeting of The Slovak Physiological Society, The Physiological Society and The Federation of European Physiological Societies
9/11/2007-9/14/2007
Bratislava, Slovakia
METABOTROPIC HORMONAL FACTORS REACT TO PROPYLENE GLYCOL SUPPLEMENTATION IN PERIPARTUM DAIRY COWS
Abstract number: PW05-35
Gyorffy1 A., Keresztes2 M., Faigl2 V., Frenyo1 V.L., Kulcsar2 M., Gaal3 T., Huszenicza2 G., Zsarnovszky1 A., Bartha1 T.
1Szent Istvan Univ. Fac. Veterinary Sci. Department of Physiology and Biochemistry
2SzIU FVS Department and Clinics of Obstetrics and Reproduction
3SzIU FVS Department and Clinic of Internal Medicine, Budapest, Hungary; [email protected]
Aims:
In peripartum dairy cows energy intake does not cover energy demands; therefore, a so-called Negative Energy Balance (NEB) evolves. Excessive NEB leads to reproductive and metabolic disorders. We aimed at investigating the effects of propylene glycol (PG) on the metabotropic hormone system.
Methods:
Experiments were conducted on Holstein cows (n1 = 49). The feed of experimental animals was supplemented with pulverous PG for 3 weeks peripartum. Blood samples were collected once a week for determination of plasma T4 and T3 concentrations. Tissue gene expression was investigated from liver biopsy samples (n2 = 16 samples obtained on d 7-10 postpartum) by means of real-time PCR and analyzed by statistical methods.
Results:
In the treated group, plasma concentrations of triiodothyronine (T3) and thyroxine (T4) were significantly (P < 0.001) higher than those found in controls. PG supplementation significantly increased the gene expression of type I deiodinase (2.4-fold increase; P = 0.001) and that of the short-form of leptin receptor (11.9-fold increase; P = 0.001). The gene expression of leptin and its long-form receptor did not change significantly.
Conclusion:
Our results indicate that PG increases the basal metabolic rate by facilitating the conversion of increased levels of T4 to T3. This idea is supported by the observed increase in DIO1 mRNA expression. Increased Ob-Ra mRNA levels suggest that leptin effects on energy homeostasis and reproduction are simultaneously also modulated.
To cite this abstract, please use the following information:
Acta Physiologica 2007; Volume 191, Supplement 658 :PW05-35