Aim: 

To investigate the influence of antagonist of AMPA/kainate glutamate receptors IEM 1751 on basal GAS in rats with intact nervous system (INS) and after the bilateral vagotomy.

Methods: 

The study was carried out in acute experiments in 46 white rats under urethane anesthesia (1.10 g/kg, intraperitoneally (i.p.)). The animals were divided into two groups: I - rats with INS; II - rats after surgical bilateral vagotomy. GAS was investigated by method of isolated stomach perfusion by Ghosh and Shild. We studied the influence of antagonist of central and peripheral AMPA/kainate glutamate receptors IEM 1751 (5 mg/kg, i.p.) on basal GAS. The nicotinic acetylcholine receptors were blocked by pentamini (3.2 mg/kg, i.p.).

Results: 

In rats with INS IEM 1751 reduced spontaneous GAS by 44%, P < 0.01. The basal GAS was inhibited by the bilateral vagotomy by 29%, p < 0.05. However in rats after vagotomy IEM 1751 enhanced the total acid secretion by 217%, p < 0.001. In these rats pentamini removed the excitatory action of IEM 1751 on basal GAS.

Conclusion: 

These results indicate that peripheral AMPA/kainate glutamate receptors realize their effects through vagal cholinergic neurotransmission in the ENS. Probably the peripheral AMPA/ kainate glutamate receptors are located on preganglionic cholinergic nerves prejunctionally or on interneurones of ENS. Thus we supposed that endogenous glutamate acting on AMPA/ kainate glutamate receptors in CNS intensify basal GAS but in ENS suppressed basal GAS. So the central and peripheral AMPA/kainate glutamate receptors play different role in regulation of basal GAS.