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Acta Physiologica 2007; Volume 190, Supplement 656
The Scandinavian Physiological Society's Annual Meeting
8/10/2007-8/12/2007
Oslo, Norway
NEUROTRANSMITTER RESPONSES IN AN IMMORTALIZED SUBMANDIBULAR SALIVARY GLAND CELL LINE FROM THE RAT
Abstract number: P38
Aure1 MH, Galtung1 HK
1Departmen of Oral Biology, Domus Odontologica, Sognsvannsveien 10 Rikshospitalet, P.O.box 1052 Blindern, NO-0316 Oslo Norway
We wished to document whether the SV40 immortalized rat submanbidular cell line had preserved their intracellular Ca2+ response to adequate neurotransmitters and determine if it would lend itself to further comparative cell physiological studies. The cell line, a gift from D Quissell, U of Colorado, was grown under standard cell culture conditions. At the time of experiments, cells were incubated with fura-2-AM (Molecular Probes, USA) following established routines. The cells were studied in a flow through chamber with an inverted microscope. As a measure of the intracellular Ca2+ concentration, fura-2 was excited at 510 nm and the ratio of emitted light at 340 and 380 nm was recorded using MetaFluor 7.1.2 software (Universal Imaging, USA). After a few minutes in an isosmotic solution (300 mOsm, pH 7.4), the cells were exposed to norepinephrine 105M, carbamoylcholine chloride 105M, ATP 104 M and substance P 107 M for 15 min and the changes in intracellular Ca2+ were measured. Exposure to all neurotransmitters induced a transient increase in the intracellular Ca2+-level (norepinephrine 185±18% carbamoylcholine chloride 426±1%, substance P 230±38%, and ATP 127±27%. The peak Ca2+-increase occurred at 11 sec (norepinephrine and carbamoylcholine chloride), 90 sec (substance P) and 18 sec (ATP) following transmitter exposure. While norepinephrine and carbamoylcholine chloride increased the Ca2+-concentration in all cells measured, substance P and ATP induced Ca2+- responses in about 50% of the measured cells. In conclusion, in this cell line there was a strong intracellular Ca2+-increase in response to norepinephrine and carbamoylcholine chloride and a variable response to ATP and substance P. Thus, this cell line has retained a normal Ca2+-response to transmitters of the autonomic nervous system.
To cite this abstract, please use the following information:
Acta Physiologica 2007; Volume 190, Supplement 656 :P38