AMPA-type receptor (AMPAR) channels are the most abundant excitatory ligand-gated receptor channels of the vertebrate central AMPA-type glutamate receptor (GluR) channels are the most abundant excitatory transmitter receptors of the central nervous system. Four subunits with different posttransscriptional modifications and flip/flop splice variants are known. In vivo they occur as tetrameric heteromeric receptors. In the present study we analyzed the time course of desensitization and resensitization kinetics of different homomeric (coassembly of splice or editing variants of one subunit) and heteromeric (coassembly of different subunits) GluR channels. We found that time constants of desensitization had intermediate values depending on the amount of cDNA of the respective subunit at all heteromeric and homomeric GluR channels tested. The same holds true for time constants of resensitization except GluR2 flip channels were coexpressed with GluR1 channels. In this case, the time constant of resensitization had values close to that of fast resensitising GluR2 flip channels, even in case of abundance of GluR1 cDNA. Additionally, rectification and deactivation of different coassemblies were anlyzed. In conclusion, the influence of GluR subunits and their variants on the physiological properties of receptor coassemblies was studied by a fast application patch