The Ca²⁺ homeostasis within cells controls many cellular processes including gene transcription, proliferation and apoptosis. Interference with Ca²⁺ signaling may induce deregulation of cell proliferation and suppression of cell death providing a basis for cancer. In human prostate cancer, a correlation between mRNA expression of the Ca²⁺ channel TRPV6 and the staging of cancer has previously been described. We have analyzed the role of TRPV6 for cell proliferation in HEK-293 cells. We show that TRPV6 increases cell proliferation of HEK-293 cells in a Ca²⁺ dependent manner. The increased proliferation correlates with increased intracellular Ca²⁺ levels without interfering with the intrinsic Ca²⁺ dependence of HEK293 cell proliferation. Low doses of econazole inhibit TRPV6 dependent Ca²⁺ signals and cell proliferation whereas BTP2, a potent inhibitor of Ca²⁺ signals and cell proliferation in T-cells, does not influence TRPV6 dependent Ca²⁺ signals or cell proliferation of HEK-293 cells. Our data demonstrate that TRPV6 increases the rate of Ca²⁺ dependent cell proliferation which is a prerequisite for its potential role in tumor progression.