The present study was designed to investigate the effects of two atypical antipsychotics, quetiapine and olanzapine on locomotor activity that is a sign of ethanol withdrawal syndrome in rats. Adult male Wistar rats were subjects. Ethanol (7.2%, v/v) was given to rats by a liquid diet for 30 days. Control rats were pair fed an isocaloric liquid diet containing sucrose as a caloric substitute to ethanol. Quetiapine (10 mg/kg), olanzapine (5 mg/kg) and saline were injected to the rats intraperitoneally 7 days after ethanol withdrawal syndrome and the last one 30 min before ethanol withdrawal testing. After 2nd hour of ethanol withdrawal, rats were observed for 5 min and withdrawal signs that included locomotor hyperactivity were recorded. Quetiapine produced significant inhibitory effects on the signs of ethanol withdrawal. Our results suggest that acute quetiapine treatment has some beneficial effects on ethanol withdrawal in rats. Thus, this drug may be useful for treatment of ethanol withdrawal syndrome.