Hypoxy-ishemic damage of brain tissue in prenatal, period considers to be one of the main causes of newborn's death. Using flow cytometry technique we examined the neuronal resistance to oxidative stress induced by preincubation of cells with H2O2. The primary culture of neurons was isolated from cerebellums of 8–10-day-old rats. Tissue slices was processed by collagenaza with following perturbation and filtering cells through teflon strainer. Experimental group consisted of rat pups born from females, subjected to acute hypoxia on the 9–10th day of gestation. The determination of active forms of oxygen was performed using the method of flow cytometry with the help of fluorescent probe - H2DCFDA (2',7'-dichlorodihydrofluorescein diacetate). The amount of dead cells in suspension was determined from the fluorescence of propidium iodide.

Active forms of oxygen content in neurons of control and experimental animals did not significantly differ. Preincubation of neuron suspension with 10 mM H2O2 for 30 min led to the significant increase of active forms of oxygen level either in experimental or in control group, though H2O2-induced enhance in the latter case was 1,5-6 times less. The increased level of active forms of oxygen was not accompanied with elevation in amount of dead cells.