Active stimulator of angiogenesis, nitric oxide is essential for the multifactorial gastric mucosal healing, because the enhanced capillary circulation facilitates the oxygen and nutrient supply to the healing site. This study was undertaken to investigate the functional role of NO-dependent mechanism of regulation in gastroprotection induced by plant flavonoids: Amaranth seed extract (ASE)and guelder rose water extract (GRWE)in acute ethanol injury on rats. Methods: Gastric lesions were induced by 100% ethanol applied intragastrically and WRS; several series of rats were used without (vehicle only) and with pretreatment with graded doses of ASE and GRWE without and with sensory denervation by neutotoxic dose of capsaicin. Lesions area was determined by planimetry. Content NO of was assessed biochemically. Results: Tested ASE and GRWE afforded gastroprotection against ethanol and WRS damage and these was accompanied by an increase in NO potent stimulator of angiogenesis and vasodilatator for gastric microcirculation, both changes being reversed by pretreatment with neurotoxic dose of capsaicin. Conclusions: NO involvement plays a critical role in angiogenesis via gastroprotection induced by plant flavonoids.