Cell migration plays a role in many (patho-)physiological processes like immune defense or tumor metastasis. The intracellular calcium concentration is an important regulator of the "migration machinery". The intracellular calcium concentration itself is amongst others controlled by the influx of calcium ions through calcium channels that belong to the family of "transient receptor potential" (TRP) channels. In HepG2 hepatoblastoma cells these channels are TRPV1 channels. Stimulating TRPV1 channels with capsaicin increases the motility of HepG2 cells. TRPV1 channels are also activated by lowering extracellular pH (pH_e). Here we tested, whether protonation of TRPV1 influences HepG2 cell migration. Cells were monitored with time lapse video microscopy. Migration was quantified from the displacement of the cell center. Cells were kept in medium of pH 6.8, pH 7.2 and pH 7.5 and pretreated with hepatocyte growth factor. The capsaicin-mediated increase of the displacement is most pronounced at pH_e7.2 (+ 88 %) while it is only 42 % and 16 % at pH_e6.8 and pH_e7.5, respectively. The stimulatory effect of capsaicin on the migratory speed which reflects fast cytoskeletal remodeling is no longer present at pH_e6.8 and pH_e7.5. In summary, our experiments reveal that the stimulatory effect of TRPV1 activation on cell migration strongly depends on pH_e.