Skin potential level (SPL) is the electric potential difference between certain sites on the surface of humane skin. It is related to the functional sate of skin (Podshibiakin, 1960), growth and regeneration (Zatsepina et al, 2004), skin barrier homeostasis (Denda & Kumazawa, 2002), local skin lesions (Uchida, 1999) and adaptive reactions of the whole organism (Gorgo, 1989; Iliukhina et al. 1995). SPL negativity at the palm area is considered to be an index of arousal level changes (Leiderman & Shapiro, 1963; Nishimura et al. 1995). We measured the SPL using a voltmeter with input resistance (50 megohm) applying Ag/AgCl electrodes to the palm and a zone of facial skin or by applying them to two facial zones. It was shown, that SPL is more positive at aural and zygomatic zones than at forehead, nasal and superciliary zones, a difference of approximately 5 mV. The SPL difference between forehead and nasal zones (FN gradient) was gender-related and varied according to the level of emotional excitement. The FN gradient was more positive in women and more negative in men. The FN gradient was more positive during emotional stress (entrance examination) than during a calm state. Although the physiological mechanisms of SPL are not entirely clear, we regard facial SPL distribution to be useful in the investigation of autonomic regulation. We thank David Shapiro and Lyudmila Didyk for critical reading the abstract.