Back
Acta Physiologica 2006; Volume 186, Supplement 650
Joint Meeting of The German Society of Physiology and The Federation of European Physiological Societies 2006
3/26/2006-3/29/2006
Ludwig-Maximilians-University, Munich
IN VITRO STUDY ABOUT NO-DEPENDENT EFFECTS OF THE HYDROSOLUBIL CIGARETTE SMOKE EXTRACT ON THE RAT'S VASCULAR AND TRACHEAL SMOOTH MUSCLE
Abstract number: PM12P-6
PANTEA1,2,3 V, Noveanu1,2,3 L, Fira-Mladinescu1,2,3 O, Mihalas1,2,3 G
1University of Medicine and Pharmacy Victor Babes, Timisoara, RomaniaMedical Student, University of Medicine and Pharmacy Victor Babes, Timisoara
2Physiology Discipline, UMPh, Timisoara
3Physiopathology Discipline, UMPh, Timisoara
For the in vitro study there were used tracheal and thoracic aorta preparations obtained from 10 Sprague-Dawley rats. The norepinephrine dose-effect curve for aortic preparations and the acetylcolin one for the tracheal ones were determined. Also, it was studied their reactivity against N^G-nitro-L-arginine (NO Sintaze inhibitor) and against sodium nitroprusside (NO donor)before and after the incubation for 1 hour with 5% hCSE. The response of the preparations against norepinephrine and respectively acetylcolin was not significantly different after the incubation with hCSE. After the administration of 10^-5M N^G-nitro-L-arginine there was a contractil response representing 20–30% of the submaximal concentration of the specific contractile agonist. This response decreased significantly (p<0.05) after the incubation. Also, the vasodilatator and bronchodilatator response decreased significantly. In conclusion, hCSE significantly decreased the "biodisponibility" of NO by decreasing the NOS activity and the smooth muscles'sensitivity against NO.
To cite this abstract, please use the following information:
Acta Physiologica 2006; Volume 186, Supplement 650 :PM12P-6