Spreading depression (SD), a pronounced self-propagating depolarization of neurons and glia, is assumed to play a role in transient neurological disorders such as migraine. This phenomenon is not only well-known in animals but also in human neocortical regions. However, it has not been studied in the human amygdala. In slices of human amygdala (lateral nucleus; 500mm; n=6) from patients (age: 13–58 years; gender 2(f), 2(m)) who underwent surgery for the treatment of refractory epilepsy, glass capillaries (10mm; field potentials) and bipolar metal electrodes (single electrical stimuli) were placed at the polar regions of the lateral nucleus. SD elicited by both KCl injection (n = 4) and reduction of the extracellular Cl- concentration (n = 2). DC fluctuation propagated at velocity of 2.6 mm / min. Field potentials evoked by single electrical stimulation were suppressed transiently during propagation of SD and reappeared within 5–15 min. Our findings show the ability of human lateral amygdala for eliciting and propagation of SD. These may contribute to transient emotional disturbances accompanying migraine in human.