Hypercholesterolemia is known to induce oxidant stress. Sulfites, which are commonly used as preservatives, have been reported to increase formation of free radicals that was accompanied by changes in visual evoked potentials (VEPs). Our study was designed to investigate the effect of sulfite treatment on VEPs of hypercholesterolemic rats. For this purpose, 3 months aged Wistar rats were divided into eight groups (n=10/group); control(C), sulfite(S), hypercholesterolemic(H), vitamin E(E), hypercholesterolemic+sulfite(HS), sulfite+vitamin E(SE), hypercholesterolemic+vitamin E(HE), hypercholesterolemic+sulfite+vitamin E(HSE). Hypercholesterolemia was established by feeding rats a %1 cholesterol diet. Sulfite (25 mg/kg) was administered to the animals via their drinking water. Vitamine E was given at dose of 50mg/kg by gastric gavage. Flash VEPs were recorded, plasma cholesterol levels were determined. Plasma cholesterol were increased in all sulfite exposed and hypercholesterolemic rats. Hypercholesterolemia caused prolongation of all VEP components(P1,N1,P2,N2,P3). Sulfite treatment increased N1, P3 latencies of VEPs in S group. Vitamine E administration reduced N2 latencies in ES group in respect to S group; also caused to shorten all latencies of HE and HSE groups in comparison with H, and HSE groups, respectively.