The trigeminal nerve is the major mediator of somatosensory perceptions from the mammalian head and comprises neurons that transduce mechanical, thermal and chemical stimuli. Individual neurons mediate sensory input from selective areas of the head. Physiological features of peripheral neurons depending on their function and area of innervation remain largely unclear. Therefore, we established a double tracing approach based on marker protein expressing variants of the Pseudorabies virus and identified trigeminal neurons (TGNs) innervating the nasal mucosa or the orbital facial skin of mice. For in vitro analysis of identified neurons, we used patch-clamp and Ca-imaging techniques and characterized nasal and cutaneous TGN for their capsaicin, menthol and ATP sensitivity as well as for expression of TTX-sensitive Na-channels. We found that both populations were similar in sensitivity to capsaicin and positive staining for IB4. Compared with TGNs innervating the nasal mucosa, a significantly lower fraction of neurons connected with the skin were sensitive to menthol. However, more cutaneous than nasal neurons responded to a P2X3 receptor specific agonist and showed expression of TTX-resistant Na-channels. These data show that TGNs with various innervation patterns differ in their sensory properties and might point to different physiological functions related to thermo-, chemo- and pain sensation.