The in vitro analysis of vascular function using organ bath systems has several limitations including the loss of blood flow, blood pressure, nerval innervations, and operative traumata. In this study we tried to circumvent these limitations by the in vivo imaging method optical coherence tomography (OCT). The vascular function of murine arteria saphena (A. saphena) of male C57BL/6 mice aged 10 weeks was analysed (n=5). The inner diameter of A. saphena (90.8±15.1 mm) constricted to 32.6±9.3 mm in response to 80 mmol/L potassium and dilated to 226.0±13.2 mm in response to 1 mmol/L sodium nitroprussid. These diameter changes happened within 45 seconds after transdermal application of vasoactive substances. The half- maximal constriction time of potassium was 7.61 seconds and comparable to 8.69 seconds of half-maximal dilation time of sodium nitroprussid. These data were combined with isometric force measurements representing 70 mmHg of control (3.05±0.33 mN/mm), 80 mmol/L potassium (10.00±2.61 mN/mm) and 1 mmol/L sodium nitroprussid (2.47±0.37 mN/mm). Using the OCT technique we were able to image the murine vascular function in vivo.