It is well-known that hypoxia and mechanical stimuli can lead to the expression of angiogenesis-regulating factors. These stimuli are essential in the formation of new blood vessels. Therefore, this study was undertaken to investigate the biological responses in this field following highly intensified stimuli (vibration and artificial normobaric hypoxia). Twelve cyclists participated in this study. Each subject completed four training sessions (normoxia without vibration, normoxia with vibration, hypoxia without vibration and hypoxia with vibration, 90 minutes cycling for each) at weekly intervals in a randomised order. For each subject five blood samples were taken at each training session (pre training, 0h, 0.5h, 1h and 4h post training). ELISA measurements showed an increase in endostatin at 0h post exercise, independently of induced stimuli. MMP-2 demonstrated a very similar time course compared to endostatin, but MMP-9 reached its maximum at 4h post exercise. VEGF was increased only at 0h post training in situations with induced vibration. Taken together, these results suggest a possible key role of endostatin in the regulation of local blood supply in the vasculature and lead to a broader understanding of angiogenic-related responses.