When expressed in Xenopus oocytes, serum- and glucocorticoid- induced kinases sgk1 and sgk3 have been shown to stimulate the activity of the sodium-dependent cotransporters for glucose (SGLT1) and dicarboxylates (NaDC1), which serve to absorb these substrates either from the intestinal chymus or from the primary urine to maintain cells with fuels. NaDC3, the basolateral sodium dicarboxylate cotransporter, is responsible for the uptake of dicarboxylates necessary for organic anion-dicarboxylate exchange via OAT1 and OAT3 that contribute to renal detoxification. We studied the impact of sgk1 and sgk3 on oocytes expressing the human NaDC3. At -60 mV, coexpression of hNaDC3 and sgk1 reduced the a-ketoglutarate-induced current from -49±20 nA to -32±23 nA, whereas coexpression with sgk3 increased the a-KG-induced current from -18±5 nA to -38± 15 nA. Coexpression with the sgks did not change the affinity for a- KG, but sgk1 reduced Imax and sgk3 increased Imax. Succinate uptake was 171±47 pmol/30min?oocyte in the absence and 74±18 pmol/30min?oocyte in the presence of sgk1. Succinate uptake increased from 56±24 pmol/30min?oocyte in the absence to 128±25 pmol/30min?oocyte in the presence of sgk3. These studies reveal differential effects of sgks on NaDC3.