Appendix 1

Bekker, R., Verweij, G.L., Bakker, J.P. & Fresco, L.F.M. (2000) Soil seed bank dynamics in hayfield succession. 88 594-608. Appendix 1. Total seed numbers and vegetation frequency of species in the dry and wet series. E: early successional species, L: late successional species, R:Rest-group, containing mainly ruderals and tree species. ! indicates target species of the successional series. L.I.: Longevity Index calculated after Thompson et al. (1997), - not available. One seed found corresponds with 7.96 seed per m². Different letters indicate significant differences of the mean seed numbers tested per species and per sampling depth using a One way ANOVA followed by a Tukey's range test or differences of mean ranks using a Kruskal-Wallis followed by a Student Newman Keuls test. * P<0.05, ** P<0.01, *** P<0.001. Species order is determined by importance in the vegetation.

		Dry series	seed bank 0-5 cm					seed bank 5-10 cm					vegetation frequency
	L.I.		A	B	C	D	sign.	A	B	C	D	sign.	A	B	C	D
E	0.29	Rumex acetosa (Rua)	59^b	21^a	20^a	7^a	***	10	0	2	1	*	100	99	99	87
E	0.56	Holcus lanatus (Hl)	392^c	211^bc	47^ab	29^a	***	51^b	38^b	4^a	8^a	***	100	98	99	91
E	0.30	Taraxacum agg.(Tagg)	0	0	0	0		1	0	0	0		100	37	57	0
E	0.65	Cerastium fontanum (Cf)	16^ab	32^b	6^ab	0^a	**	4	2	3	0		98	56	69	3
E	0.21	Anthryscus sylvestris (As)	0	0	0	0		0	0	0	0		97	12	24	0
E	0.39	Poa pratensis (Pp)	5^a	48^b	31^ab	5^a	*	6	19	7	5		78	99	79	0
E	0.20	Dactylis glomerata (Dg)	0	0	0	0		0	0	0	0		68	12	55	0
E	0.68	Ranunculus repens (Rr)	7^a	230^b	154^b	87^ab	***	1^a	83^b	104^b	70^b	***	66	98	80	6
E	0.14	Alopecurus pratensis (Ap)	0	0	0	0		0	0	0	0		55	0	0	0
E	0.0	Rhinanthus angustifolius (Rha)	0	0	0	0		0	0	0	0		51	47	27	0
E	0.55	Veronica arvensis (Va)	7	4	2	0		2	1	2	0		25	0	0	0
E	0.75	Stellaria media (Sm)	0	0	0	0		0	0	0	0		13	5	1	0
E	0.49	Cardamine pratensis (Cpra)	0	0	0	0		0	0	0	0		12	12	0	0
E	0.0	Holcus mollis (Hm)	0	0	0	0		0	0	0	0		11	24	15	5
E	0.20	Lolium perenne (Lp)	0	0	0	0		0	0	0	0		9	0	1	0
E	0.33	Bromus hordeaceus (Bh)	0	0	0	0		0	0	0	0		5	5	0	0
E	0.08	Festuca pratensis (Pp)	0	0	0	0		0	0	0	0		1	24	2	0
E	0.67	Rumex obtusifolius (Ro)	0^a	19^a	910^b	0^a	***	0^a	3^a	964^b	0^a	***	1	7	32	0
E	0.40	Trifolium repens (Tr)	0	1	0	0		0	3	1	2		1	1	3	0
E	-	Epilobium spp. (Esp)	1	4	1	0		0	0	0	0		1	0	0	0
E	0.73	Alopecurus geniculatus (Ag)	0	0	0	0		0	0	0	0		0	25	0	1
E	0.24	Galium aparine (Ga)	0	0	0	0		0	0	0	0		0	12	5	0
E	0.27	Deschampsia cespitosa (Dc)	0	0	0	0		0	0	0	0		0	12	0	0
E	0.54	Glyceria fluitans (Gf)	0	0	0	2		0	0	0	0		0	11	0	0
E	0.17	Glechoma hederacea (Gh)	0	0	3	0		0	0	2	0		0	0	13	0
E	0.28	Phleum pratense	0	2	2	0		0	1	0	0		0	0	0	0
E	0.90	Poa annua (Pann)	5^a	63^b	5^a	1^a	***	26^b	54^b	0^a	0^a	***	0	0	0	0

L	-	Agrostis spp.(Asp)	9^a	255^ab	435^bc	942^c	***	1^a	116^b	149^b	80^b	***	55	99	100	100
L	0.14	Festuca rubra (Fr)	0	0	0	0		0	0	0	0		0	53	100	100
L	0.29	Anthoxanthum odoratum (Ao)	0^a	2^a	2^a	12^b	**	0	0	0	0		1	72	98	100
L!	0.37	Luzula campestris (Lc)	1	0	0	3		0	0	0	0		1	0	15	85
L	-	Carex spp.(Csp)	1^a	79^b	4^a	193^b	***	1^a	20^ab	1^a	66^b	***	0	22	4	42
L	0.70	Juncus acutiflorus (Ja)	0^a	0^a	9^ab	26^b	**	0	7	12	5	*	0	0	0	20
L	0.32	Hypochaeris radicata (Hr)	0	0	0	0		0	0	0	0		0	0	13	11
L	0.35	Plantago lanceolata (Pl)	0	1	3	0		0	0	0	0		9	60	59	0
L	0.35	Stellaria graminea (Sg)	0	0	0	0		0	0	0	0		1	11	41	0
L	0.62	Stellaria uliginosa	3	1	3	0		4	3	0	0		0	0	0	0
L	0.45	Ajuga reptans (Ar)	1	0	2	3		1	0	1	2		0	0	0	0
L!	0.88	Calluna vulgaris	1	0	0	0		8^b	0^a	1^ab	2^ab	*	0	0	0	0

R	-	Juncus bufonius-group	136	4877	190	8402		184	2501	295	1465		0	69	0	72
R	0.78	Urtica dioica (Ud)	14	3	10	0		11	3	15	1		16	0	0	0
R	0.30	Alnus glutinosa	19^b	6^ab	23^b	1^a	***	1	0	4	0		0	0	0	0
R	-	Betula spp.(Bsp)	9^a	17^a	30^ab	118^b	**	1	1	4	12		0	2	2	6
R	-	Salix spp.	1	2	3	0		1^a	0^a	12^b	1^a	*	0	0	0	0
R	0.91	Gnaphalium uliginosum	0	0	1	2		2	1	0	1		0	0	0	0
R	0.24	Lythrum salicaria	0	5	2	0		0	2	0	0		0	0	0	0
R	-	Rubus spp.(Rsp)	0	0	0	0		0	1	0	0		0	0	5	0
R	0.46	Galeopsis tetrahit (Gt)	0	0	1	0		0	0	0	0		0	0	7	0
R	0.87	Sagina procumbens	0	0	0	3		0	0	0	1		0	0	0	0
R	-	Rorippa palustris	0	0	0	4		0	0	0	0		0	0	0	0
R	0.0	Quercus robur (Qr)	0	0	0	0		0	0	0	0		11	13	8	38
		total number of species	19	22	26	18		19	19	19	16		27	29	29	16
		total number of individuals	687	5883	1899	9840		316	2859	1583	1722

Wet series			seed bank 0-5 cm					seed bank 5-10 cm					vegetation frequency
	L.I.		A	B	C	D	sig.	A	B	C	D	sig.	A	B	C	D
E	0.56	Holcus lanatus (Hl)	718^c	36^a	300^b	162^b	***	182^b	37^a	164^b	7^a	***	100	100	100	100
E	0.68	Ranunculus repens (Rr)	480^b	739^c	694^bc	98^a	***	380^a	668^a	1433^b	389^a	***	100	100	100	100
E	0.39	Poa pratensis (Pp)	138	154	259	199		134^ab	208^b	150^ab	54^a	**	100	98	100	99
E	-	Agrostis spp.(Asp)	524^c	33^ab	105^bc	8^a	***	579^c	83^ab	167^b	4^a	***	100	34	93	0
E	0.49	Cardamine pratensis (Cpra)	83	71	56	38		58a^c	55^a	95^b	96^b	**	94	100	100	100
E	0.73	Alopecurus geniculatus (Ag)	0	0	0	0		0	0	0	0		90	2	0	0
E	0.08	Festuca pratensis (Fp)	0	0	1	0		0	0	0	0		76	95	93	50
E	0.0	Rhinanthus angustifolius (Rha)	0	0	0	0		0	0	0	0		75	100	98	97
E	0.54	Glyceria fluitans (Gf)	59^ab	111^bc	293^c	31^a	**	97^a	264^b	1316^c	344^b	***	68	8	76	3
E	0.29	Rumex acetosa (Rua)	30^b	5^a	5^a	4^a	**	16^b	0^a	2^a	1^a	**	64	100	95	100
E	0.20	Lolium perenne (Lp)	0	0	0	0		0	0	0	0		45	2	0	0
E	0.30	Taraxacum agg. (Tagg)	9^b	0^a	0^a	1^a	*	4^b	0^a	0^a	0^a	*	23	53	14	45
E	0.90	Poa annua (Pann)	11^b	7^ab	0^a	0^a	*	38^b	12^b	1^a	2^a	*	2	5	0	0
E	0.65	Cerastium fontanum (Cf)	73	24	25	7		65^b	11^a	8^a	3^a	*	0	56	17	70
E	-	Epilobium spp. (Esp)	2	4	3	5		0	5	3	2		0	53	100	3
E	0.40	Trifolium repens (Tr)	0	0	0	0		0	0	0	0		0	43	16	9
E	0.25	Potentilla anserina(Pans)	0	0	0	0		0	3	0	0		0	31	0	0
E	0.27	Deschampsia cespitosa (Dc)	0	0	13	0		0	0	21	1		0	15	98	0
E	0.16	Leontodon autumnalis (La)	0	0	0	0		0	0	0	0		0	10	4	0
E	0.23	Mentha aquatica (Ma)	0	0	3	1		0	0	0	3		0	9	0	0
E	0.75	Stellaria media (Sm)	0	25	9	0		1^a	32^b	1^a	0^a	**	0	6	0	1
E	0.14	Alopecurus pratensis (Ap)	0	0	0	0		0	0	0	0		0	1	0	0
E	0.55	Veronica arvensis (Va)	1	0	0	2		0^a	0^a	0^a	6^b	*	0	0	0	0
E	-	Callitriche spp.	0^a	21^c	40^bc	2^ab	**	3^a	49^b	46^ab	2^a	**	0	0	0	0
E	0.67	Rumex obtusifolius (Ro)	1	0	4	2		1	2	2	2		0	0	0	0
E	0.28	Phleum pratense	4^ab	0^a	36^b	2^a	*	0^a	0^a	6^b	0^a	*	0	0	0	0
E	0.29	Veronica scutellata	0	0	0	1		0^a	0^a	0^a	7^b	**	0	0	0	0
E	-	Lycopus europaeus	0	0	1	2		0	0	2	3		0	0	0	0

L!	0.29	Anthoxanthum odoratum (Ao)	0^a	1^a	30^b	78^b	***	0^a	0^a	13^b	11^b	**	0	97	100	100
L	0.35	Plantago lanceolata (Pl)	0^a	0^a	11^b	96^b	***	0	0	0	0		1	97	100	99
L!	0.11	Caltha palustris (Cpal)	0^a	1^ab	0^a	12^b	*	0^a	0^a	0^a	11^b	***	0	15	5	95
L!	0.70	Juncus acutiflorus (Ja)	29^a	203^b	40^a	631^b	***	79^a	233^b	57^a	770^b	***	0	65	6	92
L	0.02	Cynosurus cristatus (Cc)	0	0	0	0		0	0	0	0		0	55	62	88
L	0.32	Trifolium pratense (Tp)	0	0	0	0		0	0	0	0		0	8	11	56
L!	0.56	Lychnis flos-cuculi (Lf)	0^a	4^a	1^a	108^b	***	0^a	5^a	1^a	137^b	***	0	25	1	54
L	0.11	Filipendula ulmaria (Fu)	0	0	0	0		0	0	0	0		0	11	4	52
L!	-	Carex spp. (Csp)	1	4	9	13		2^a	27^b	29^b	16^b	*	0	15	71	49
L	0.26	Cirsium palustre (Cip)	0	0	0	1		0	0	2	0		0	2	7	40
L	0.14	Festuca rubra (Fr)	0	0	0	0		0	0	0	0		0	0	7	40
L	0.26	Galium palustre (Gp)	0	0	0	0		0	0	2	4		0	18	23	38
L!	-	Lotus uliginosus (Lu)	0	0	0	0		0	0	0	0		0	21	4	30
L	0.15	Myosotis palustris (Mp)	0^a	9^b	2^a	11^ab	*	0^a	42^b	6^ab	31^ab	*	4	44	12	19
L	0.45	Ajuga reptans (Ar)	0	5	0	0		1^a	6^b	0^a	0^a	*	0	1	0	18
L	0.62	Stellaria uliginosa (Su)	15^ab	27^bc	734^c	1^a	**	15^ab	43^b	157^c	0^a	***	0	14	0	8
L	0.12	Galium uliginosum (Gu)	0	0	0	0		0^b	8^a	0^b	0^b	**	0	5	0	3
L	0.63	Ranunculus flammula (Rf)	0^a	21^b	17^b	8^ab	*	2^a	44^b	15^a	6^a	***	0	0	0	2

R	-	Juncus bufonius-group	1568	8485	11373	4134		1171	5450	7165	4774		21	60	84	49
R	-	Betula spp. (Bsp)	23^b	3^a	1^a	2^a	*	10^b	0^a	2^a	1^a	*	0	0	2	0
R	1.0	Lythrum portula	7	2	1	0		7^b	0^a	2^ab	0^a	*	0	0	0	0
R	-	Salix spp.	8^b	8^b	0^a	0^a	*	5^b	5^b	0^a	0^a	*	0	0	0	0
R	0.91	Gnaphalium uliginosum	4^a	15^b	9^ab	0^a	*	6^ab	7^bc	32^c	1^a	*	0	0	0	0
R	0.30	Alnus glutinosa	8	1	3	0		2	2	0	0		0	0	0	0
R	0.78	Urtica dioica	5	2	3	0		2	4	7	3		0	0	0	0
R	-	Rorippa palustris	2	5	1	0		1	6	1	1		0	0	0	0
R	0.87	Sagina procumbens (Sp)	0^a	43^b	26^a	3^a	*	1^a	28^b	6^a	4^a	**	0	1	0	0
R	0.35	Polygonum hydropiper	0	0	0	0		0^a	0^a	13^b	1^a	*	0	0	0	0
		total number of species	25	30	32	30		27	28	33	32		16	40	31	32
		total number of individuals	3803	10069	14108	5663		2862	7339	10927	6697