Supplementary material to Sorensen A.-M., Kröber S., Ulrike S. U., Huijser P., Dekker K. and Heinz S. The Arabidopsis ABORTED MICROSPORES (AMS) gene encodes a MYC class transcription factor. The Plant Journal 2002; 32: 413–423.

Figure S1.  Antisense (a,c,e) and sense (b,d,e) in situ analysis of the AMS transcript.

(a) Post-meiotic anthers showing a low signal of the AMS transcript in the tapetum (arrow) and early microspores (arrowhead).

(b) Successive section to that shown in (a), probed with the AMS sense probe.

(c) Free microspores (arrow) and tapetal tissue (arrowhead) showing a slight increase in AMS signal.

(d) Successive section to that shown in (c), probed with the AMS sense probe. The pollen has a higher background, possibly due to the nature of the developing pollen wall.

(e) Mature microspores and anther wall show no detectable AMS signal.

Successive section to that shown in (e), probed with the AMS sense probe. Here, the pollen and anther wall stains brown as in the previous antisense section shown in (e). Bar = 100 mm.

This figure is available for download as a TIFF file here.