In vitro properties of blood bank produced fibrin sealants from single donor human plasma
Abstract number: PP-TH-210
Eshghi1 P., Hashemi2 A., Amirizadeh2 N., Abolghasemi3 H.
11Pediatric Hematology and Oncology, Shahid Beheshti University of medicine 22Research center of Iranian Blood Transfusion Organization 33General Director, Iranian Blood Transfusion Organization, Tehran, Iran (Islamic Republic of)
How-to-cite Eshghi P, Hashemi A, Amirizadeh N, Abolghasemi H. In vitro properties of blood bank produced fibrin sealants from single donor human plasma. Journal of Thrombosis and Haemostasis 2009; Volume 7, Supplement 2: Abstract PP-TH-210
Background and Objective: Blood bank produced fibrin sealants(FS) are more accepted than commercial ones, because their production has lower cost and- in the case of autologous FSs- has no risk of transfusion transmitted diseases and hypersensitivity in comparison with pooled plasma derived FSs.However their efficacy, biomedical,mechanical,concenteration and activity of their main hemostatic proteins should be assesed and documented.
Material and method: Twenty units of human Fresh Frozen Plasma(FFP) and Cryopercipitate were used as the sources of fibrinogen(FI) and thrombin(FIIa)respectedly.FI was precipitated in different temperatures by Protamine sulfate and its concentration was assayed with Enzyme-linked Immunosorbant and Clauss clotting methods.FIIa was extracted from FFP by adding ethanol and calcium chloride in a tube containing glass granules and Kaoline and its activity was determined using specific chromogenic substrate by spectrophotometry.Clotting time(CT) was measured following mixture of FIIa and FI.Tensile strength(TS) Adhesion Strength(AS)of FS were evaluated with some special designed devices. Clot stability was measured by Clot lysis time in 5 M Urea.
Results: Mean FI concetration measured by Elisa and Clauss clotting method were 73 ± 7 and 71 ± 7 mg/mL respectedly(%93 recovery)with highest precipitation in 24C.Mean FIIa activity was 59.6 NIH and it was stable untill 6 h at 4°C,2 h at room temperature and 3 months at-28°C.Mean clotting time was 5 s.Mean TS and AS were 60 ± 8.9 g/cm2 and 55 g respectedly.Antifibrinolytic agents had no effect on CT and TS but improved clot lysis time when added to FI.
Conclusion: FI and FIIa prepared with this method from single human plasma units have appropriate characteristics for production of FS.
Disclosure of interest: none declared.
To cite this abstract use the following format:
Journal of Thrombosis and Haemostasis 2007; Volume 5, Supplement 2: abstract number
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