Whole blood thromboelastometry in human and cynomolgus monkeys
Abstract number: PP-WE-248
Spiezia1 L., Bertini1 D., Boldrin2 M., Gavasso1 S., Radu1 C., Zerbinati1 P., Bulato1 C., Ancona3 E., Cozzi2 E., Simioni1 P.
11Cardiologic, Thoracic, and Vascular Sciences 22CORIT 33Department of Surgical and Gastrointestinal Sciences, University of Padova, Padova, Italy
How-to-cite Spiezia L, Bertini D, Boldrin M, Gavasso S, Radu C, Zerbinati P, Bulato C, Ancona E, Cozzi E, Simioni P. Whole blood thromboelastometry in human and cynomolgus monkeys. Journal of Thrombosis and Haemostasis 2009; Volume 7, Supplement 2: Abstract PP-WE-248
Introduction: To clarify the features of blood coagulation in primate, rotation thromboelastometry (ROTEM) profile was compared between monkeys and a group of healthy humans. Moreover, comparison with traditional clotting parameters were performed in a subgroup of primates.
Materials and Methods: Four ROTEM¯ (INTEM, EXTEM, FIBTEM, and NATEM) assays in 40 naïve cynomolgus monkeys and 50 healthy human volunteers were performed. The main parameters considered were: clotting time (CT), clot formation time (CFT), maximum clot firmness (MCF), and alpha-angle. In a subgroup of 22 animals standard coagulative profiles were performed.
Results: CT and CFT mean values were statistically significant shorter in primates than humans. Moreover, alpha-angle and MCF were higher in primates than humans (Table 1). Hct, fibrinogen, PS, and AT mean levels were lower in monkeys than humans. On the contrary Plts count, FVIII, PC, were statistically significant higher in monkeys.
Table 1
| | Monkey (n = 40) | Human (n = 50) | | Monkey (n = 0) | Human (n = 50) |
|---|
CT (s)
INTEM | 138 ± 3 | 177 ± 4∗ | alphaangle (°) | 82 ± 1 | 73 ± 1∗ |
| EXTEM | 55 ± 2 | 85 ± 20∗ | 80 ± 1 | 70 ± 1∗ | |
| FIBTEM | 51 ± 2 | 67 ± 3∗ | 74 ± 1 | 65 ± 1∗ | |
| NATEM | 405 ± 18 | 689 ± 17∗ | 69 ± 1 | 47 ± 1∗ | |
CFT (s)
INTEM | 40 ± 1 | 84 ± 3∗ | MCF (mm) | 70 ± 1 | 57 ± 1∗ |
| EXTEM | 52 ± 2 | 103 ± 3∗ | 64 ± 1 | 56 ± 1∗ | |
| FIBTEM | – | – | 19 ± 1 | 12 ± 1∗ | |
| NATEM | 111 ± 7 | 258 ± 11∗ | 63 ± 1 | 48 ± 1∗ | |
| Monkeys versus Human ∗P value < 0.0001. |
Conclusion: ROTEM¯ profile shows a higher hypercoagulable profile in primates as compared to humans. In xenotransplantation hypercoagulable state might be a more different issue than in human to be overcome.
Disclosure of interest: none declared.
