Congress of the International Society on Thrombosis and Haemostasis

Inhibition of PAI-1 production by treatment of herbal medicine in human umbilical vein endothelial cells

Abstract number: PP-MO-864

ATSUMI¹ G., Ohkura¹ N., Kurata¹ A., Kanai¹ S., Fukuda² T., Matsuda¹ J.

¹¹Faculty of pharmaceutical sciences, Teikyo University, Sagamihara ²²Health Sciences R&D Lab., POLA Chemical Industries, Inc., Yokohama, Japan

How-to-cite ATSUMI G, Ohkura N, Kurata A, Kanai S, Fukuda T, Matsuda J. Inhibition of PAI-1 production by treatment of herbal medicine in human umbilical vein endothelial cells. Journal of Thrombosis and Haemostasis 2009; Volume 7, Supplement 2: Abstract PP-MO-864

The flower of Prunus persica BATSCH has been used for ethonomedical to induce bowel movement and diuretic action. Although Prunus flowers have been shown to improve the blood flow by the avoidance of thrombogenesis in the traditional Chinese herbal medicine, the mechanism of this effect is not clear. To elucidate this mechanism, we examined whether Prunus flower extracts can inhibit plasminogen activator inhibitor 1 (PAI-1) production in human umbilical vein endothelial cells (HUVEC). HUVEC were stimulated by TNFa in the presence of Prunus flower extracts for 12 h. After stimulation, culture medium was collected and PAI-1 amounts were measured by ELISA. Using 70% ethanol extracted fraction, dose-dependent decrease of PAI-1 production was observed. Further fractionation was attempted from Prunus flower 70% ethanol extract, and inhibitory effects were found in the both butanol and water extracted fractions equivalently, but not in the hexane and ethyl acetate extracted fractions. To identify active compounds, water extracted fraction was separated by normal phase HPLC and two active compounds, HT-1 and HT-2 were obtained. Furthermore, another two active materials, HT-4 and HT-5, were also isolated from butanol extracted fraction by normal phase HPLC. The structure of these four materials were characterized as, prunasinic acid, neoprunasinic acid, multiflorin B and multinoside A, respectively.

Disclosure of interest: none declared.

To cite this abstract use the following format:

Journal of Thrombosis and Haemostasis 2007; Volume 5, Supplement 2: abstract number

Session Details

Date:	Unpresented
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Session name:	ISTH2009
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