A NOVEL COMPLETE HIGH SHEAR RATE ARTERIAL THROMBOSIS AND BLEEDING MODEL IN BABOONS

Abstract number: P-W-454

Roodt¹ J.P., Lamprecht¹ S., Silence² K., Ulrichts² H., Dreier² T., Meiring¹ S.M., Badenhorst¹ P.N.

¹¹Haematology and Cell Biology, University of the Free State, Bloemfontein, South Africa ²²Ablynx NV, Zwijnaarde, Belgium

How-to-cite Roodt JP, Lamprecht S, Silence K, Ulrichts H, Dreier T, Meiring SM, Badenhorst PN. A NOVEL COMPLETE HIGH SHEAR RATE ARTERIAL THROMBOSIS AND BLEEDING MODEL IN BABOONS. J Thromb Haemost 2007; 5 Supplement 2: P-W-454

Abstract

Introduction: Given the high incidence of cardiovascular events, there is a need for safer and more effective antithrombotic therapies that have to be tested in predictive and relevant animal models. The aim of this study was to develop a complete, technically uncomplicated arterial thrombosis and bleeding model in baboons.

Methods: The femoral artery of baboons was shunted to the femoral vein. The artery was mechanically injured and external stenosis was applied. This resulted in thrombus formation measured by decreased blood flow. Thrombi were mechanically dislodged resulting in a pattern of cyclic flow reductions (CFRs). Escalating doses of Aspirin, Clopidogrel, heparin or Abciximab were infused and the effect of this treatment on the CFRs was measured. The effect of these drugs in an incisional and a template bleeding model were assessed.

Results: Aspirin and heparin failed to prevent thrombosis while Clopidogrel and Abciximab efficiently prevented CFRs. Infusion of epinephrine reversed the inhibition of CFRs by Clopidogrel, but not with Abciximab. Blood loss from a well defined wound correlated better with clinical bleeding observations than template bleeding times.

Conclusions: The efficacy of the tested drugs in this model closely matches the clinical situation in man, suggesting that our model is relevant for the human situation, and can assess the efficacy and safety in terms of induced bleeding tendency in one study. Less animals are needed to obtain relevant data with this model, as each animal can be used twice.

References:

To cite this abstract use the following format:

Journal of Thrombosis and Haemostasis 2007; Volume 5, Supplement 2: abstract number

Session Details

Date:	01/08/2007
Time:	00:00-00:00
Session name:	XXIst ISTH Congress
Subject:
Location:	Oxford, UK
Presentation type:
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