Back
A FUNCTIONAL AUTOMATED ASSAY FOR THROMBOMODULIN ACTIVITY IN PLASMA
Abstract number: P-W-074
Rousseau1 A., Van Dreden1 P., Woodhams1 B.J.
1R and D, Stago, Gennevilliers, France
How-to-cite Rousseau A, Van Dreden P, Woodhams BJ. A FUNCTIONAL AUTOMATED ASSAY FOR THROMBOMODULIN ACTIVITY IN PLASMA. J Thromb Haemost 2007; 5 Supplement 2: P-W-074
Abstract
Introduction: Thrombomodulin (TM), a marker of endothelial cell injury, plays an important role in the protein C system and in activation of TAFI. Current routine assays of TM are ELISA antigen assays methods. No routine activity assay is available. The aim of this study was to validate a simple assay for the determination of TM activity (TMa) in plasma.
Methods: TMa levels were measured on the STA-R analyser (Stago, France) using a prototype chromogenic assay based on the ability TMa to activate protein C after incubating with FIIa, Protein C, polybrene and a fibrin polymerisation inhibitor. The activity is monitored with an APC substrate (CBS 4246) at 405nm. The test was evaluated in apparently healthy volunteers and in a number of selected patient groups associated with increased levels of TM (diabetes, cancers, sepsis, multiple trauma).
Results: This prototype assays is automated on the STA-R. Intra assays and inter assays CVs are < 4% and < 5% respectively. No activity standard is available so all results are expressed as a % of normal pool. The assay gives a linear response between 0 and 200%. The assay is insensitive to low molecular weight or unfractionated heparin (<1IU) and endogenous protein C, FIIa or TAFI. The mean normal range was102%16 (n=43). The levels of TMa found in patients with diabetes (n=15), cancers (n=10), sepsis (n=12) and multiple trauma (n=20) were 130%28, 155%60, 173%56 and 185% 62 respectively, all were statistically higher than normal (p< 0.05).
Conclusions: This prototype automated assay allows rapid determination of TMa levels in plasma. The preliminary clinical study shows a significant increase in level in the patient groups tested. Further studies are required to confirm these findings, but this could be a useful assay in the routine laboratory for determination of TMa.
To cite this abstract use the following format:
Journal of Thrombosis and Haemostasis 2007; Volume 5, Supplement 2: abstract number
Session Details
| Date: |
01/08/2007
|
| Time: |
00:00-00:00
|
| Session name: |
XXIst ISTH Congress |
| Subject: |
|
| Location: |
Oxford, UK |
| Presentation type: |
|
| Back to top |
|
