APPLICABILITY OF A THROMBIN GENERATION TEST IN LABORATORY SCREENING
Abstract number: P-T-112
King1 V., Grimm1 L., Pusateri1 A.E.
1Physiology, Novo Nordisk Research US, New Brunswick, United States
How-to-cite King V, Grimm L, Pusateri AE. APPLICABILITY OF A THROMBIN GENERATION TEST IN LABORATORY SCREENING. J Thromb Haemost 2007; 5 Supplement 2: P-T-112
Introduction: The calibrated automated thrombogram (CAT) is used extensively in research to monitor coagulation, and there is growing interest in its clinical use. It was of interest to relate CAT parameters to the standard screening assays PT and aPTT.
Methods: CAT was performed on platelet poor human plasma using the 5 pM tissue factor reagent. Lag time (Lag), endogenous thrombin potential (ETP), peak thrombin (Peak), and time to Peak (TTP) were determined. Automated PT and aPTT were performed. Local normal ranges (2.5-97.5%) were determined on 70 normal samples from other labs. 20 abnormal (PT/aPTT) samples were also assayed. All 90 samples were re-categorized based on local ranges.
Results: With prolonged PT and aPTT, Lag was extended in 6 of 12; ETP was reduced in 7 of 12; Peak was reduced in 11 of 12; and TTP was prolonged in 6 of 12. With prolonged PT only, Lag was extended in 1 of 8; ETP was reduced in 1 of 8; Peak was reduced in 7 of 8; and TTP was extended in 0 of 8. With prolonged aPTT only, Lag was extended in 1 of 2; ETP was reduced in 0 of 2; Peak was reduced in 0 of 2; and TTP was extended in 1 of 2. With normal PT and aPTT, Lag and TTP were normal in 68 of 68, and ETP and Peak were normal 67 of 68.
|*Correlation of aPTT with Lag P = 0.05; others P < 0.01.|
Conclusions: ETP and Peak were most highly correlated with PT and aPTT. With both PT and aPTT abnormal, Peak was most often reduced (92% of the time). With both screens normal, all CAT parameters were normal 98% of the time, suggesting that normal screening tests are a reasonable indicator for normal thrombin generation. CAT may be most applicable as a secondary test following abnormal screening assays.
To cite this abstract use the following format:
Journal of Thrombosis and Haemostasis 2007; Volume 5, Supplement 2: abstract number
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