A COMPARATIVE EVALUATION OF THE CS-2000I COAGULATION ANALYSER USING CLOTTING, AMIDOLYTIC AND IMMUNO-TURBIDOMETRIC ASSAYS
Abstract number: P-T-089
Lawrie1 A.S., Kobayashi2 K., Tajima2 K., Hoshiko2 S., Matsuo2 N., Hamaguchi2 Y., Liesner3 R., Machin1 S.J., Mackie1 I.J.
11Haemostasis Research Unit, Department of Haematology, University College London, London, United Kingdom 22Research & Development, Sysmex Corporation, Kobe, Japan 33Haematology Department, University College Hospitals NHS Trust, London, United Kingdom
How-to-cite Lawrie AS, Kobayashi K, Tajima K, Hoshiko S, Matsuo N, Hamaguchi Y, Liesner R, Machin SJ, Mackie IJ. A COMPARATIVE EVALUATION OF THE CS-2000I COAGULATION ANALYSER USING CLOTTING, AMIDOLYTIC AND IMMUNO-TURBIDOMETRIC ASSAYS. J Thromb Haemost 2007; 5 Supplement 2: P-T-089
Abstract
Introduction: The CS-2000i, a new coagulation analyser from Sysmex, is capable of performing coagulation, amidolytic and immuno-turbidometric assays. Transmitted light can be monitored at multiple wavelengths (340, 405, 575, 660, 800nm), allowing more efficient clot detection in the presence of interfering substances. The CS-2000i also offers a pre-analytical check and flagging system for haemolysis, icterus and lipaemia (HIL check).
Methods: We evaluated this new analyser against the CA-1500 (light scatter) and STA Compact (mechanical) devices, using 200 samples from normal subjects and patients with icteric (bilirubin <=489umol/L), lipaemic (cholesterol <=8mmol/L, triglycerides <=7.5mmol/L), or haemolysed plasmas (plasma Hb 0.4-6.1g/L), and high or low fibrinogen content (>5 or <0.5g/L).
Results: The CS-2000i was able to detect excessive icterus and haemolysis at 405 & 575nm respectively and potentially flag these sample problems. Biphasic clot waveforms could be detected in some samples. Between day assay imprecision (n=10x5) was low for clotting tests (PT, APTT, Clauss Fg) with normal (cv <0.83%) and abnormal (cv <2.39%) plasmas; chromogenic assays (PC, AT-III, Plg) with normal (cv <1.49%) and abnormal (cv <3.37%) plasmas; immuno- assays (D-Dimer) with 560ug/L (cv 2.32%) and 2500ug/L (cv 2.24%) plasmas. PT (INR 1.0-5.0), APTT (27-115s) and Fg (0.5-12.5g/L) showed good correlations with the CA-1500 (r=1.00; 0.99 & 0.99 respectively) and STA Compact (r=1.00; 0.97 & 0.98, respectively). PC (15-270u/dL), AT-III (20-180u/dL) and Plg (17-200 u/dL) showed good correlations with the CA-1500 (r=1.00; 0.99 & 0.98 respectively). D-Dimer (0-1500ug/L) showed good correlations with the CA-1500 (r=0.95).
Conclusions: A new clot detection algorithm avoided early reaction errors and the facility to switch analysis to different wavelengths avoided interference by HIL checking, while fibrinogen sensitivity was excellent. While chromogenic and immuno-turbidometric assays exhibited good correlation with the CA-1500 analyser.
