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A COMPARATIVE ANALYSIS OF THE RESPONSE TO INFLAMMATORY STIMULI OF BLOOD- AND MESENCHYMAL STEM CELLDERIVED ENDOTHELIAL PROGENITOR CELLS
Abstract number: P-M-358
Liu1 J.W., Bounameaux1 H., Kruithof1 E.K.O.
1Internal Medicine, University Hospital of Geneva, Geneva, Switzerland
How-to-cite Liu JW, Bounameaux H, Kruithof EKO. A COMPARATIVE ANALYSIS OF THE RESPONSE TO INFLAMMATORY STIMULI OF BLOOD- AND MESENCHYMAL STEM CELL–DERIVED ENDOTHELIAL PROGENITOR CELLS. J Thromb Haemost 2007; 5 Supplement 2: P-M-358
Abstract
Introduction: The use of "endothelial progenitor cells" (EPC) for therapy of ischemic cardiovascular disease is under intense investigation. Three different cell types have been proposed for such therapy: early outgrowth EPC, late outgrowth (LO)-EPC and the Mesenchymal Stem Cell-derived Endothelial-like cells (MSCE). The most suitable cell type for each clinical application still has to be established. Recently, we have isolated and characterized MSCE and shown that these cells can be readily obtained in large numbers from adult bone marrow. In addition, MSCE stimulate and participate in new blood vessel formation in vivo. Because inflammation is an important determinant of cardiovascular disease, we compared the response of LO-EPC and MSCE to inflammatory stimuli.
Methods: LO-EPC and MSCE were obtained from human cord blood and human adult bone marrow respectively and incubated with TNF (10 ng/ml), IL-1 (50 ng/ml), or lipopolysaccharide (100 ng/ml). Cellular responses to these inflammatory stimuli were measured by flow cytometry analysis of the leukocyte adhesion molecules VCAM-1 and E-selectin.
Results: Treatment with TNF, IL-1 or LPS resulted in a marked increase in expression of VCAM-1 and E-selectin. In contrast, in MSCE, no increase in expression of these proteins was seen after treatment with these inflammatory stimuli. The lack of response to TNF is due to the absence of the relevant receptors TNFR1 or TNFR2.
Conclusions: MSCE are resistant to inflammatory activation by TNF, IL-1 or LPS, whereas blood derived LO-EPC are readily activated. In view of their resistance to inflammation and their easier availability in large numbers from adult sources, MSCE may provide an interesting cell type for autologous cell based-therapy of ischemic disease. This may be particularly relevant in ischemic disease with a strong inflammatory component.
To cite this abstract use the following format:
Journal of Thrombosis and Haemostasis 2007; Volume 5, Supplement 2: abstract number
Session Details
| Date: |
01/08/2007
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| Time: |
00:00-00:00
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| Session name: |
XXIst ISTH Congress |
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| Location: |
Oxford, UK |
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