INFLAMMATION, ENDOTHELIAL DYSFUNCTION AND ANGIOGENIC ACTIVITY IN STABLE HEART FAILURE PATIENTS
Abstract number: P-S-437
Reganon1 E., Vila1 V., Martínez Sales1 V., Almenar2 L., Sánchez Lázaro2 I., Villa3 P.
11Research Center 22Cardiology Service 33Clinical Pathology Department, Universitary Hospital La Fe, Valencia, Spain
How-to-cite Reganon E, Vila V, Martínez Sales V, Almenar L, Sánchez Lázaro I, Villa P. INFLAMMATION, ENDOTHELIAL DYSFUNCTION AND ANGIOGENIC ACTIVITY IN STABLE HEART FAILURE PATIENTS. J Thromb Haemost 2007; 5 Supplement 2: P-S-437
Introduction: Accumulating evidence indicate that inflammatory cytoquines and non cytoquines, angiogenic, angiostatic and endothelial activity mediators have an important role in the pathogenesis of heart failure (HF), contributing to cardiac remodelling and peripheral vascular disturbances. This study was conducted to assess the relationship between circulating markers of inflammation, endothelial dysfunction and angiogenic activity in stable HF patients
Methods: We studied 59 patients with stable HF of more than one years duration, 69% NYHA Class I/II and 31% NYHA class III/IV, and 59 age and sex-matched healthy controls circulating levels of fibrinogen (turbidity assay), sialic acid (SA) (colorimetric enzymatic method), C-reactive protein (CRP) (immunological agglutination technique), interleukin 6 (IL-6) (ELISA) tumour necrosis factor-alpha (TNF-a) (ELISA), von Willebrand factor (VWF) (ACL 9000 coagulation analyzed), vascular endothelium growth factor (VEGF) (ELISA) and thrombospondin (TSP) (indirect ELISA) were determined.
Results: Patients had higher levels of fibrinogen (30667 vs. 24943 mg/dl, p<0.001), SA (6112 vs. 579 mg/dl, p<0.05), CRP (7.812 vs. 1.72.1 mg/L, p<0.001), TNF-a (1.71.2 vs. 0.90.3 pg/ml, p<0.001), IL-6 (6.315 vs. 1.81.5 pg/ml, p<0.05), VEGF (260275 vs. 167137 pg/ml, p<0.05) and VWF (194 103 vs. 10451, p< 0.001) and lower levels of TSP (4013 vs. 4713 mg/ml, p< 0.01) than controls. The OR of the parameters studied showed that increased concentrations of CRP (OR=18.4), TNF-a (OR=15.7), VWF (OR=13.9) and fibrinogen (OR=11.9) and decreased concentrations of TSP (OR=9.1) were the parameters with the largest effect in HF.
Conclusions: Stable HF is associated with and increased inflammatory markers levels and endothelial dysfunction. Increased VEGF and decreased TSP concentrations suggest angiogenic activity closely related to endothelial integrity after injury in HF.
FIS, PI020176. CEUiC, GVA: 05081