CIRCULATING ENDOTHELIAL PROGENITOR CELLS AND INFLAMMATION IN PATIENTS BEFORE AND AFTER CARDIAC SURGERY
Abstract number: P-S-410
Cesari1 F., Caporale2 R., Marcucci1 R., Gori1 A., Capalbo3 A., Alamanni1 C., Spinelli1 A., Stefano4 P., Abbate1 R., Gensini5 G.
11Medical and Surgical Critical Care, Thrombosis Centre, University of Florence 22Central Laboratory, Azienda Ospedaliero-Univeristaria Careggi 33Medical and Surgical Critical Care, Thrombosis Centre, Institute of Sports Medicine 44Department of Heart and Vessels, Unit of Cardiac Surgery, Azienda Ospedaliero-Univeristaria Careggi 55Medical and Surgical Critical Care, Thrombosis Centre, Don Carlo Gnocchi Foundation, Florence, Italy
How-to-cite Cesari F, Caporale R, Marcucci R, Gori A, Capalbo A, Alamanni C, Spinelli A, Stefano P, Abbate R, Gensini G. CIRCULATING ENDOTHELIAL PROGENITOR CELLS AND INFLAMMATION IN PATIENTS BEFORE AND AFTER CARDIAC SURGERY. J Thromb Haemost 2007; 5 Supplement 2: P-S-410
Abstract
Introduction: Endothelial progenitor cells (EPCs) are bone marrow-derived progenitor cells with the capacity to home to sites of vascular injury. Aim of the study was to assess EPCs' changes in relation to inflammatory markers in patients undergoing cardiac surgery.
Methods: In 38 CABG (group A) and in 54 patients who underwent a valve replacement (group B) EPCs, IL6, IL8, IL10, IL1Ra, VEGF, high sensitivity CRP and NT-ProBNP levels were determined pre-surgery (T1), at 3 (T2) and 10-15 days after surgery (T3). Peripheral blood EPCs were defined as CD34+KDR+, CD133+KDR+ and CD34+CD133+KDR+ by flow cytometry.
Results: EPCs did not significantly differ between group A and B at T1 and at T2. At T2, EPCs significantly decreased both in group A and B [CD34+KDR+: 0.24 (0-2.69) cells/ml vs 0.14 (0-0.75) cells/ml; CD133+KDR+: 0.23 (0-2.65) cells/ml vs 0.12 (0-0.68) cells/ml; CD34+CD133+KDR+: 0.17 (0-2.93) vs 0.10 (0-0.57)] cells/ml. Moroever, a significant increase of all cytokines and NT-ProBNP was observed between T1 and T2 in both groups, whereas a significant inverse correlation between of EPCs' number and NT-ProBNP after cardiac surgery was detected (CD34+/KDR+: r= -0.22, p=0.04; CD133+/KDR+: r=-0.24, p=0.03; CD34+/CD133+/KDR+: r=-0.25, p=0.02). At T3 EPCs significantly increased with respect to T2 in both groups [CD34+KDR+: 0.14 (0-0.75) cells/ml vs 0.27 (0-1.65) cells/ml; CD133+KDR+: 0.12 (0-0.68) cells/ml vs 0.25 (0-1.09) cells/ml; CD34+CD133+KDR+: 0.10 (0-0.57) vs 0.17 (0-1.58)] cells/ml. At a multiple linear regression model IL-10 and IL1ra were reported to be significant predictors of EPCs at T3.
Conclusions: In conclusion, we documented a significant association between cardiac surgery and modifications of EPCs' number, by showing, for the first time, that IL-1ra and IL-10 are significant predictors of the EPCs' number at T3. Furthermore, our findings suggest a role for NT-pro BNP in reducing the number of circulating EPCs.
