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A NEW ANIMAL MODEL OF CATHETER-SELECTIVE MIDDLE CEREBRAL ARTERY THROMBO-OCCLUSIVE STROKE: DEMONSTRATION OF RAPID REPERFUSION BY LOCAL PLASMIN INFUSION
Abstract number: P-S-400
Jahan1 R., Stewart2 D., Vinters3 H.V., Vinuela1 F., Vandeberg4 P., Marder2 V.J.
11Radiological Sciences 22Hematology 33Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles 44Biochemistry, Talecris Biotherapeutics, Research Triangle Park, United States
How-to-cite Jahan R, Stewart D, Vinters HV, Vinuela F, Vandeberg P, Marder VJ. A NEW ANIMAL MODEL OF CATHETER-SELECTIVE MIDDLE CEREBRAL ARTERY THROMBO-OCCLUSIVE STROKE: DEMONSTRATION OF RAPID REPERFUSION BY LOCAL PLASMIN INFUSION. J Thromb Haemost 2007; 5 Supplement 2: P-S-400
Abstract
Introduction: Pre-clinical assessment of thrombolytic agents for stroke treatment relies on animal models, but translation of animal observations to human stroke has not been invariably predictive. We have developed a reliable new rabbit stroke model that is anatomically relevant to human stroke and which provides angiographic documentation of vascular occlusion and recanalization. We have applied the model to assess the thrombolytic efficacy of intra-arterial infusion of human plasmin [Talecris Biotherapeutics].
Methods: Following right femoral artery isolation and sheath insertion, a microcatheter was guided fluoroscopically to the common carotid artery, followed by selective placement at the terminal bifurcation of the internal carotid artery (ICA). Selective thrombo-occlusion of the middle cerebral artery (MCA) was achieved by slow infusion of a thrombin:thromboplastin solution, as documented by immediate and 2 hour followup contrast angiography. Regional plasmin infusion of 1 or 2 mg in 5 mL of unbuffered solution at acid pH was performed over 10-30 minutes into the distal ICA and thrombolysis was evaluated by angiography.
Results: Immediate occlusion of the target vessel (MCA) was verified in 7 of 7 (100%) animals, with no spontaneous thrombolysis or recanalization evident on followup angiography two hours later. Recanalization was achieved in 3 of 3 plasmin-treated animals, within 30 minutes using 1 mg plasmin and within 10 minutes using 2 mg plasmin, as documented by angiography. Cerebral infarction was demonstrated histologically at necropsy.
Conclusions: We describe a new model of MCA ischemic stroke in the rabbit which provides reliable, selective thrombo-occlusion and cerebral infarction in 7 of 7 animals, and which was utilized to demonstrate successful plasmin-induced recanalization in 3 of 3 animals. This model is relevant for human MCA ischemic stroke and applicable for definitive angiographic assessment of thrombolytic efficacy and safety.
To cite this abstract use the following format:
Journal of Thrombosis and Haemostasis 2007; Volume 5, Supplement 2: abstract number
Session Details
| Date: |
01/08/2007
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| Time: |
00:00-00:00
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| Session name: |
XXIst ISTH Congress |
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| Location: |
Oxford, UK |
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