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A COMPARISON OF TWO COMMERCIAL ADAMTS-13 ACTIVITY ASSAYS WITH A REFERENCE LABORATORY METHOD
Abstract number: P-S-282
Crist1 R.A., Rodgers2 G.M.
11R and D, Hemostasis and Thrombosis, ARUP Institute for Clinical and Experimental Pathology 22Departments of Pathology and Medicine, University of Utah Medical Center, Salt Lake City, UT, United States
How-to-cite Crist RA, Rodgers GM. A COMPARISON OF TWO COMMERCIAL ADAMTS-13 ACTIVITY ASSAYS WITH A REFERENCE LABORATORY METHOD. J Thromb Haemost 2007; 5 Supplement 2: P-S-282
Abstract
Introduction: ADAMTS-13 is the enzyme responsible for cleaving the ultra large von Willebrand factor multimers. Deficiency of ADAMTS-13 is associated with thrombotic thrombocytopenic purpura (TTP), a serious disorder usually diagnosed with clinical criteria. Previous test methods used to measure ADAMTS-13 activity were time consuming and technically challenging. Recently, commercial kits have been developed which use fluorescence resonance energy transfer (FRET) methodology to measure ADAMTS-13 activity. In this study, we compared results from two recently released commercial kits with results obtained from a reference laboratory method using FRET technology to assay samples from patients referred for a possible diagnosis of TTP.
Methods: Method comparison testing was performed using two commercial assay kits: ADAMTS-13 Activity Assay (GTI, Waukesha, Wisconsin, USA) and the Technozym ADAMTS-13 ELISA kit (Technoclone, Vienna, Austria). Tests were read on a SpectraMax M5 microplate reader (Molecular Devices Corp., Sunnyvale, California, USA). Sodium citrate plasma specimens were tested on both commercial methods according to the assay protocol, and data was analyzed using Microsoft Excel spreadsheets obtained from each kit manufacturer. All three assays report results as a percentage of normal.
Results: A total of 29 samples were assayed and compared. Six samples had less than 5% of normal activity identified by the reference laboratory. The GTI kit identified four of these six samples, while the Technoclone kit identified all six. The correlation coefficient comparing the reference laboratory vs. GTI was 0.82. The correlation coefficient comparing the reference laboratory vs. Technoclone was 0.91.
Conclusions: In this comparison study, the Technoclone ADAMTS-13 activity kit identified 100% of the severe deficiency samples, while the GTI kit identified 67%. The availability of reliable ADAMTS-13 assays will improve the rapid and accurate diagnosis of TTP.
To cite this abstract use the following format:
Journal of Thrombosis and Haemostasis 2007; Volume 5, Supplement 2: abstract number
Session Details
| Date: |
01/08/2007
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| Time: |
00:00-00:00
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| Session name: |
XXIst ISTH Congress |
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| Location: |
Oxford, UK |
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