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PROGNOSTIC SIGNIFICANCE OF MRD (MINIMAL RESIDUAL DISEASE) QUANTIFICATION BY PCR IN CHILDHOOD ACUTE LEUKEMIA
Abstract number: P-S-239
Elbaz1 O., Mansour2 A., Abd El-Kader3 A., El-Menshawi3 N., El-Demery4 A.
11Hematology, Faculty of medicine, Mansoura 22Pediatrics 33Hematology 44Oncology, Faculty of medicine, mansoura, Egypt
How-to-cite Elbaz O, Mansour A, Abd El-Kader A, El-Menshawi N, El-Demery A. PROGNOSTIC SIGNIFICANCE OF MRD (MINIMAL RESIDUAL DISEASE) QUANTIFICATION BY PCR IN CHILDHOOD ACUTE LEUKEMIA. J Thromb Haemost 2007; 5 Supplement 2: P-S-239
Abstract
Introduction: TEL- AML1 t(12;21)(p13:q22) is the most frequent genetic rearrangement in acute lymphoblastic leukemia.
Methods: Fifty five cases with ALL(including 10 relapsing ones) were included in this study. They received induction, intensification and maintenance chemotherapy. Also, they received cranial irradiation to a total dose of 1800 cGY in 2 weeks (10 fractions). Again, intrathecal chemotherapy was given. Determination of MRD Was done using real time PCR to detect t(12:21) (p13: q22) TEL-AML1.
Results: It was found that TEL-AML1 was positive in 36.79% of the newly diagnosed cases with a mean ratio of 0.560.29 while in relapsed patients, it was positive in 33.3% of cases with a mean ratio of 0.850.16. Again, it was found that patients with good response to chemotherapy showed a lower TEL-AML1 ratio (0.02-0.28) while those with slow or bad response to chemotherapy showed higher ratios(0.420.1 and 0.760.14 respectively). A positive correlation was found between TEL-AML1 ratio and hepatomegaly, splemegaly, lymphadenopthy and FAB stage classification. On the other hand, no correlation between TEL-AML1 ratio and other hematological parameters e.g.haemoglobin, total leucocyric coun, platelet count and blast cell mass.
Conclusions: Quantification of TEL-AML1 may play a role in predicting the response to chemotherapy in cases with ALL.
References: Moppett T, Burke G, Steward C, Oakhill A and Goulden N (2003):- J. of Clinical Patholog; 56:249.
To cite this abstract use the following format:
Journal of Thrombosis and Haemostasis 2007; Volume 5, Supplement 2: abstract number
Session Details
| Date: |
01/08/2007
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| Time: |
00:00-00:00
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| Session name: |
XXIst ISTH Congress |
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| Location: |
Oxford, UK |
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