Soluble VEGF/sFlt-1 Ratio is Independent Predictor of AML Patient Outcome

Abstract number: P0675

Aref¹ S, El Sherbiny² M, Goda¹ T, Fouda¹ M, Al Askalany¹ H, Abdalla¹ D

¹¹Mansoura University, Mansoura, Egypt ¹¹Mansoura University, Mansoura, Egypt ²²Cairo University, Cairo, Egypt

Angiogenesis is controlled by a balance between positive and negative angiogenic regulatory factors. In the present work, we tested the differential prognostic relevance of soluble vascular endothelial growth factor (VEGF), their receptors 1 (Flt-1), 2 (KDR), and the ratio between sVEGF/sFlt-1 in 43 AML patients. sVEGF and its soluble receptors were assessed using an ELISA. Soluble VEGF, sFlt-1, sKDR concentration levels was significantly higher in AML patients at diagnosis as compared to that in normal control. sVEGF, sFlt1 and sVEGF/sFlt1 ratio were significantly higher in non responders as compared to responders(P < 0.001 for all). However, there is no significant difference regarding sKDR levels (P > 0.05). sVEGF, and sVEGF/sFlt1 ratio and not sFlt1, sKDR levels were significantly elevated in non survivors as compared to survivors. sVEGF, sFlt1 levels were significantly correlated to WBCs counts (R= 0.93, P= 0.000, R= 0.56, P= 0.000 respectively); bone marrow blast cell counts (R= 0.92, P= 0.000; R= 56, P= 0.000 respectively); peripheral blood blast cell counts(R= 0.91, P= 0.000; R= 0.52, P= 0.000, R= 0.37, P= 0.014 respectively); but sKDR was only correlated to peripheral blood blast cell counts (R= 0.37, P= 0.014). Cox regression analysis results with sVEGF, sFlt1, sKDR, sVEGF/sFlt1 ratio suggest that the most important predictor for AML outcome is sVEGF/sFlt1 ratio. In conclusion: sVEGF/sFlt-1 ratio is independent predictor of AML patient out come, and is preferred to be assessed before the decision to use antiangiogenic therapy.